Embryos derived from single pronucleus are suitable for preimplantation genetic testing.

Autor:	Lebovitz O; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel. Electronic address: lebovitzo@gmail.com., Noach-Hirsh M; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Taieb S; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Haas J; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Zilberberg E; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Nahum R; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Orvieto R; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel; The Tarnesby-Tarnowski Chair for Family Planning and Fertility Regulation at the Tel Aviv Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel., Aizer A; Department of Obstetrics and Gynecology, IVF Unit, Chaim Sheba Medical Center, Tel- Hashomer, Israel; Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
Jazyk:	angličtina
Zdroj:	Fertility and sterility [Fertil Steril] 2024 Oct; Vol. 122 (4), pp. 598-606. Date of Electronic Publication: 2024 May 23.
DOI:	10.1016/j.fertnstert.2024.05.152
Abstrakt:	Objective: To study and compare the preimplantation genetic testing for monogenic disorders (PGT-M) results, and to evaluate the treatment cycle outcomes of embryos derived from a single pronucleus (1PN) vs. two pronuclei (2PN). Design: A retrospective cohort study from January 2018 to December 2022 involving in vitro fertilization (IVF)-PGT-M treatment cycles. Setting: Single, academically affiliated fertility center. Patients: A total of 244 patients underwent 351 IVF-PGT-M treatment cycles. Intervention: Embryo biopsy with molecular testing for a monogenic disorder. Main Outcome Measures: The molecular diagnosis results and clinical outcomes after the transfer of embryos derived from 1PN and 2PN in IVF-PGT-M treatment cycles. Results: Embryos derived from 1PN have a significantly low developmental potential with a lower rate of embryos that underwent biopsy compared with 2PN-derived embryos; 1PN-derived embryos demonstrated a significantly lower number of blastocysts (24% vs. 37.9%) and top-quality blastocysts (22.3% vs. 48.1%) compared with 2PN-derived embryos. Lower successfully completed and unaffected PGT-M results were achieved in 1PN compared with 2PN-derived embryos (47.1% vs. 65.5% and 18.7% vs. 31.6%, respectively), with significantly higher abnormal molecular results (39.6% vs. 22.7%). The embryo transfer of 24 1PN-derived embryos with no affected genetic disorder resulted in 5 (20.8%) clinical pregnancies and 4 (16.7%) live births (LBs). Conclusions: Within the limits of fewer embryos derived from 1PN that yielded unaffected embryos suitable for transfer, the clinical pregnancy and LB rate of 1PN embryos undergoing PGT-M are reassuring. We, therefore, suggest applying PGT-M to embryos derived from 1PN embryos to improve the cumulative clinical pregnancy and LB rates. Competing Interests: Declaration of Interests O.L. has nothing to disclose. M.N.-H. has nothing to disclose. S.T. has nothing to disclose. J.H. has nothing to disclose. E.Z. has nothing to disclose. R.N. has nothing to disclose. R.O. has nothing to disclose. A.A. has nothing to disclose. (Copyright © 2024 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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