Use of coenzyme Q-10 to improve the pregnancy rate in sheep.

Autor: Tironi SMT; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil., Sitó-Silva L; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil., de Camillo BL; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil., Denadai R; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil., Silva ALAD; Animal Science Department, Esalq-USP, State Universitu of São Paulo, Piracicaba, Brazil., de Paula Freitas-Dell'Aqua C; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil., Junior JAD; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil., de Oliveira RA; Biostatistics Department, Biosciences Institute, Paulista State University, Botucatu, Brazil., Souza MIL; Morphophysiology Department, Biosciences Institute, Federal University of Mato Grosso do Sul, Campo Grande, Brazil., Oba E; Veterinary Surgery and Animal Reproduction Department, College of Veterinary Medicine and Animal Science, Paulista State University, Botucatu, Brazil. Electronic address: eunice.oba@unesp.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2024 Jul; Vol. 266, pp. 107498. Date of Electronic Publication: 2024 May 14.
DOI: 10.1016/j.anireprosci.2024.107498
Abstrakt: One of the factors responsible for less pregnancy rates is the use of frozen semen in sheep due to the oxidative stress created by the process. The aim of this experiment was to test the effects of adding coenzyme Q-10 (CoQ10) to the seminal extender on sperm quality and the pregnancy rate of sheep. In this study, ejaculates from eight Dorper rams of reproductive age were used and tested in four treatments: Control (pure BotuBov®), C1 (175 µM of CoQ10), C3 (350 µM of CoQ10), and C7 (700 µM of CoQ10). Samples were collected in triplicate from each animal, and sperm analysis was performed by CASA after thawing at 0 h and 2 h. The samples were also analyzed by flow cytometry for plasma and acrosomal membrane integrity, stability, lipid peroxidation, mitochondrial potential, and superoxide anion production. In total, 198 ewes were inseminated by laparoscopy and divided into two groups: control (n=98) and C7 (n=100). Pregnancy diagnosis was performed at 30 days. Coenzyme Q10 proved to be safe for semen cryopreservation, not altering sperm kinetic values between the groups post-thawing. In flow cytometry, the C1 and C7 groups achieved a better index of plasma membrane integrity and membrane stability (P<0.05). A increased pregnancy rate was observed in C7 (52 %) compared to the control (38 %). In conclusion, coenzyme Q10 assists in the cryopreservation process, protecting the sperm cell and improving pregnancy rates in ewes.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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