Ultrarobust stable ABTS radical cation prepared using Spore@Cu-TMA biocomposites for antioxidant capacity assay.

Autor: Yan H; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China., Hou W; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China., Lei B; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China., Liu J; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China; Henan Province Key Laboratory for Animal Food Pathogens Surveillance, Henan Agricultural University, Zhengzhou, 450046, China., Song R; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China; Henan Province Key Laboratory for Animal Food Pathogens Surveillance, Henan Agricultural University, Zhengzhou, 450046, China., Hao W; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China; Henan Province Key Laboratory for Animal Food Pathogens Surveillance, Henan Agricultural University, Zhengzhou, 450046, China., Ning Y; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China., Zheng M; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China., Guo H; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China., Pan C; College of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450000, China. Electronic address: pancm7570@163.com., Hu Y; National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: yongganghu@mail.hzau.edu.cn., Xiang Y; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Henan Agricultural University, Zhengzhou, 450046, China; Henan Province Key Laboratory for Animal Food Pathogens Surveillance, Henan Agricultural University, Zhengzhou, 450046, China; College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450046, China. Electronic address: yuqiangxiang@henau.edu.cn.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Aug 15; Vol. 276, pp. 126282. Date of Electronic Publication: 2024 May 23.
DOI: 10.1016/j.talanta.2024.126282
Abstrakt: Herein, spore@Cu-trimesic acid (TMA) biocomposites were prepared by self-assembling Cu-based metal-organic framework on the surface of Bacillus velezensis spores. The laccase-like activity of spore@Cu-TMA biocomposites was enhanced by 14.9 times compared with that of pure spores due to the reaction of Cu 2+ ions with laccase on the spore surface and the microporous structure of Cu-TMA shell promoting material transport and increasing substrate accessibility. Spore@Cu-TMA rapidly oxidized and transformed 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) into ABTS ●+ without using H 2 O 2 . Under optimum conditions, the ABTS ●+ could be stored for 21 days at 4 °C and 7 days at 37 °C without the addition of any stabilizers, allowing for the large-scale preparation and long-term storage of ABTS ●+ . The ultrarobust stable ABTS ●+ obtained with the use of Cu-TMA could effectively reduce the "back reaction" by preventing the leaching of the metabolites released by the spores. On the basis of these findings, a rapid, low-cost, and eco-friendly colorimetric platform was successfully developed for the detection of antioxidant capacity. Determination of antioxidant capacity for several antioxidants such as caffeic acid, glutathione, and Trolox revealed their corresponding limits of detection at 4.83, 8.89, and 7.39 nM, respectively, with linear ranges of 0.01-130, 0.01-140, and 0.01-180 μM, respectively. This study provides a facile way to prepare ultrarobust stable ABTS ●+ and presents a potential application of spore@Cu-TMA biocomposites in food detection and bioanalysis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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