| Autor: |
Ivashchenko IuD, Osipova LA, Gut IT, Garmanchuk LV |
| Jazyk: |
ruština |
| Zdroj: |
Eksperimental'naia onkologiia [Eksp Onkol] 1985; Vol. 7 (6), pp. 47-9. |
| Abstrakt: |
The first step in the isolation of murine epidermal growth factor (EGF) from submaxillary glands was the trace enrichment of the acid-ethanol extract on Sep-pak C18 cartridges using the step elution with ethanol. The fraction eluted by 60% ethanol contained above 75% of EGF. The final purification of EGF was achieved by reverse-phase liquid chromatography on Novapak C18 column in the gradient of acetonitrile. A comparison of the effectiveness of gel filtration (on Bio-gel P-60 column) and trace enrichment (on Sep-pak C18 cartridges) of the appropriate fractions were analyzed by the reverse-phase high-pressure liquid chromatography, which had demonstrated that hydrophobic separation of EGF had a number of advantages. The homogeneous character of EGF was confirmed by the silver staining of gels after disc-electrophoresis and isoelectric focusing (pI 4.8). The pronounced mitogenic effect of EGF on the primary culture of the rat hepatocytes is described. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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