| Autor: |
Borba PB; Instituto Gonçalo Moniz, FIOCRUZ, Salvador 40296-710, BA, Brazil., Lago J; Serviço de Imunologia, HUPES-UFBA, Salvador 40110-060, BA, Brazil., Lago T; Serviço de Imunologia, HUPES-UFBA, Salvador 40110-060, BA, Brazil., Araújo-Pereira M; Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER Initiative), Salvador 41720-200, BA, Brazil.; Instituto de Pesquisa Clínica e Translacional (IPCT), UniFTC, Salvador 41720-200, BA, Brazil., Queiroz ATL; Instituto Gonçalo Moniz, FIOCRUZ, Salvador 40296-710, BA, Brazil.; Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER Initiative), Salvador 41720-200, BA, Brazil.; Instituto de Pesquisa Clínica e Translacional (IPCT), UniFTC, Salvador 41720-200, BA, Brazil., Barud HS; Laboratório de BioPolímeros e Biomateriais, Uniara, Araraquara 14801-340, SP, Brazil., Carvalho LP; Instituto Gonçalo Moniz, FIOCRUZ, Salvador 40296-710, BA, Brazil.; Serviço de Imunologia, HUPES-UFBA, Salvador 40110-060, BA, Brazil.; INCT-Instituto de Investigação em Doenças Tropicais, Salvador 40110-040, BA, Brazil., Machado PRL; Serviço de Imunologia, HUPES-UFBA, Salvador 40110-060, BA, Brazil.; INCT-Instituto de Investigação em Doenças Tropicais, Salvador 40110-040, BA, Brazil., Carvalho EM; Instituto Gonçalo Moniz, FIOCRUZ, Salvador 40296-710, BA, Brazil.; Serviço de Imunologia, HUPES-UFBA, Salvador 40110-060, BA, Brazil.; INCT-Instituto de Investigação em Doenças Tropicais, Salvador 40110-040, BA, Brazil., de Oliveira CI; Instituto Gonçalo Moniz, FIOCRUZ, Salvador 40296-710, BA, Brazil.; INCT-Instituto de Investigação em Doenças Tropicais, Salvador 40110-040, BA, Brazil. |
| Abstrakt: |
Leishmaniasis, caused by Leishmania parasites, is a neglected tropical disease and Cutaneous Leishmaniasis (CL) is the most common form. Despite the associated toxicity and adverse effects, Meglumine antimoniate (MA) remains the first-choice treatment for CL in Brazil, pressing the need for the development of better alternatives. Bacterial NanoCellulose (BNC), a biocompatible nanomaterial, has unique properties regarding wound healing. In a previous study, we showed that use of topical BNC + systemic MA significantly increased the cure rate of CL patients, compared to treatment with MA alone. Herein, we performed a study comparing the combination of a wound dressing (BNC or placebo) plus systemic MA versus systemic MA alone, in CL caused by Leishmania braziliensis . We show that patients treated with the combination treatment (BNC or placebo) + MA showed improved cure rates and decreased need for rescue treatment, although differences compared to controls (systemic MA alone) were not significant. However, the overall time-to-cure was significantly lower in groups treated with the combination treatment (BNC+ systemic MA or placebo + systemic MA) in comparison to controls (MA alone), indicating that the use of a wound dressing improves CL treatment outcome. Assessment of the immune response in peripheral blood showed an overall downmodulation in the inflammatory landscape and a significant decrease in the production of IL-1a ( p < 0.05) in patients treated with topical BNC + systemic MA. Our results show that the application of wound dressings to CL lesions can improve chemotherapy outcome in CL caused by L. braziliensis . |
