| Autor: |
Melaku M; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Department of Animal Science, College of Agriculture, Woldia University, Woldia P.O. Box 400, Ethiopia., Su D; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Zhao H; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Zhong R; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Ma T; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Yi B; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Chen L; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China., Zhang H; State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China. |
| Jazyk: |
angličtina |
| Zdroj: |
Biology [Biology (Basel)] 2024 May 01; Vol. 13 (5). Date of Electronic Publication: 2024 May 01. |
| DOI: |
10.3390/biology13050317 |
| Abstrakt: |
In this study, a commercial sodium butyrate protected by a new buffer salt solution (NSB) was tested to determine whether it can be used as an antibiotic alternative in broiler production. A total of 192 1-day-old broilers were randomly allocated to three dietary treatments: soybean meal diet (CON), antibiotic diet (ANT, basal diet + 100 mg/kg aureomycin), and NSB (basal diet + 800 mg/kg NSB). The growth performance, serum anti-inflammatory cytokines, intestinal morphology, gut barrier function, antioxidative parameters, SCFAs' content, and cecal microbiota were analyzed. The result showed that NSB significantly improved ADFI and ADG ( p < 0.01), and decreased FCR ( p < 0.01). Serum anti-inflammatory cytokine IL-10 was up-regulated ( p < 0.01), and pro-inflammatory TNF-α was down-regulated ( p < 0.05) by NSB supplementation. H&E results showed that VH and the VH/CD ratio significantly increased ( p < 0.05) in the jejunum and ileum in the NSB group. Furthermore, ZO-1 ( p < 0.01), claudin-1 ( p < 0.01), and occludin ( p < 0.05) in the jejunum and claudin-1 ( p < 0.01) and mucin-2 ( p < 0.05) in the ileum were significantly up-regulated in the NSB group. Additionally, SOD ( p < 0.05) and the T-AOC/MDA ratio ( p < 0.01) in the jejunum and SOD in the ileum were significantly increased ( p < 0.05) in the NSB group. The MDA level also significantly increased ( p < 0.01) in the ANT group in the jejunum. Propionic acid ( p < 0.05) and butyric acid ( p < 0.01) content significantly increased in the NSB group in the jejunum and ileum segments. The 16S rRNA sequencing results showed no significant difference ( p > 0.05) in alpha and beta diversity among the groups. LEFSe analysis also indicated that Peptostreptococcaceae , Colidextribacter , Firmicutes , Oscillospira , and Erysipelatoclostridiaceae , which promote SCFA production ( p < 0.05), were identified as dominant taxon-enriched bacterial genera in the NSB group. The Spearman correlation analysis revealed that Colidextribacter with ADFI, ADG, VH, claudin-1 ( p < 0.05), and unclassified_f__Peptostreptococcaceae with ADFI, IL-10, and ZO-1 were positively correlated ( p < 0.05). Furthermore, ADFI and ADG with IL-10, claudin-1, SOD, T-AOC, and butyric acid ( p < 0.05), and similarly, ADG with VH ( p < 0.05), showed a positive correlation. In conclusion, NSB enhanced the growth performance by improving jejunum and ileum morphology, and serum anti-inflammatory cytokines, and by regulating the intestinal barrier function and antioxidant capacity, SCFAs' content, and cecum microbiota, showing its potential use as an alternative to antibiotics in poultry nutrition. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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