| Autor: |
Dos Santos Oliveira D; BioMark@ISEP-CEB/LABBELS, School of Engineering, Polytechnic Institute of Porto, R. Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal.; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal., Oliveira ASR; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal., Mendonça PV; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal., Coelho JFJ; Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, Rua Sílvio Lima, 3030-790 Coimbra, Portugal.; Instituto Pedro Nunes (IPN), Associação para a Inovação e Desenvolvimento em Ciência e Tecnologia, R. Pedro Nunes, 3030-199 Coimbra, Portugal., Moreira FTC; BioMark@ISEP-CEB/LABBELS, School of Engineering, Polytechnic Institute of Porto, R. Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal., Sales MGF; BioMark@ISEP-CEB/LABBELS, School of Engineering, Polytechnic Institute of Porto, R. Dr. António Bernardino de Almeida, 4249-015 Porto, Portugal.; BioMark@UC-CEB/LABBELS, Department of Chemical Engineering, Faculty of Sciences and Technology, University of Coimbra, Pole II, R. Sílvio Lima, 3030-790 Coimbra, Portugal. |
| Abstrakt: |
This work presents a novel approach for tailoring molecularly imprinted polymers (MIPs) with a preliminary stage of atom transfer radical polymerization (ATRP), for a more precise definition of the imprinted cavity. A well-defined copolymer of acrylamide and N , N '-methylenebisacrylamide (PAAm-co-PMBAm) was synthesized by ATRP and applied to gold electrodes with the template, followed by a crosslinking reaction. The template was removed from the polymer matrix by enzymatic/chemical action. The surface modifications were monitored via electrochemical impedance spectroscopy (EIS), having the MIP polymer as a non-conducting film designed with affinity sites for CA15-3. The resulting biosensor exhibited a linear response to CA15-3 log concentrations from 0.001 to 100 U/mL in PBS or in diluted fetal bovine serum (1000×) in PBS. Compared to the polyacrylamide (PAAm) MIP from conventional free-radical polymerization, the ATRP-based MIP extended the biosensor's dynamic linear range 10-fold, improving low concentration detection, and enhanced the signal reproducibility across units. The biosensor demonstrated good sensitivity and selectivity. Overall, the work described confirmed that the process of radical polymerization to build an MIP material influences the detection capacity for the target substance and the reproducibility among different biosensor units. Extending this approach to other cancer biomarkers, the methodology presented could open doors to a new generation of MIP-based biosensors for point-of-care disease diagnosis. |
