| Autor: |
Fang Y; Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 24205, Taiwan., Lin SY; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Orthopaedic Research Centre, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Regenerative Medicine and Cell Therapy Research Centre, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; School of Post-Baccalaureate Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Department of Orthopedics, Kaohsiung Medical University Gangshan Hospital, Kaohsiung 820, Taiwan., Chen CH; Department of Orthopaedic Surgery, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Orthopaedic Research Centre, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Regenerative Medicine and Cell Therapy Research Centre, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Department of Orthopaedics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 80145, Taiwan.; Ph.D. Program in Biomedical Engineering, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan.; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan.; Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan., Lo HC; Department of Nutritional Science, Fu Jen Catholic University, New Taipei City 24205, Taiwan. |
| Abstrakt: |
Acute pancreatitis (AP) is characterized by elevated intracellular Ca 2+ concentrations, mitochondrial dysfunction, and oxidative stress in pancreatic acinar cells. Algal oil (AO) has demonstrated antioxidant and anti-inflammatory properties. This study aims to explore the effects of algal oil on the microenvironment of AP. Rat pancreatic acinar AR42J cells were pretreated with AO containing 0, 50, 100, or 150 μM of docosahexaenoic acid (DHA) 2 h prior to AP induction using sodium taurocholate (STC). After 1 h of STC treatment, AR42J cells exhibited a significant increase in intracellular Ca 2+ concentration and the production of amylase, lipase, reactive oxygen species, and pro-inflammatory mediators, including tumor necrosis factor-α and interleukin-6. These STC-induced increases were markedly reduced in cells pretreated with AO. In comparison to cells without AO, those treated with a high dose of AO before STC exposure demonstrated a significant increase in mitochondrial membrane potential and a decrease in lipid peroxidation. Furthermore, STC-activated nuclear factor kappa-B (NF-κB) was attenuated in AO-pretreated cells, as evidenced by a significant decrease in activated NF-κB. In conclusion, AO may prevent damage to pancreatic acinar cells by alleviating intracellular Ca 2+ overload, mitigating mitochondrial dysfunction, reducing oxidative stress, and attenuating NF-κB-targeted inflammation. |
