| Autor: |
Fawzy EM; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt., Selim MA; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt., Mostafa NE; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt., Abdelhameed RM; Department of Applied Organic Chemistry, National Research Centre, Dokki, Giza, Egypt., Darwish AM; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt., Yousef AM; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt., Alabiad MA; Department of Pathology, Faculty of Medicine, Zagazig University, Egypt., Ibrahim MN; Department of Clinical Laboratories, College of applied Medical Sciences, Jouf University, Qurrayat77451, KSA., Fawzy HM; Department of Community, Faculty of Medicine, Zagazig University, Sharkia, Egypt., Abdel Hamed EF; Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Sharkia, Egypt. |
| Abstrakt: |
Background: Inflammatory bowel disease is an autoimmune disease that affects the gut. T. spiralis larvae (E/S Ags) loaded on calcium-benzene-1,3,5-tricarboxylate metal-organic frameworks (Ca-BTC MOFs) were tested to determine whether they might prevent or cure acetic acid-induced murine colitis. Methods: T. spiralis larvae E/S Ags/Ca-BTC MOFs were used in prophylactic and therapeutic groups to either precede or follow the development of murine colitis. On the seventh day after colitis, mice were slaughtered. The effect of our target antigens on the progress of the colitis was evaluated using a variety of measures, including survival rate, disease activity index, colon weight/bodyweight, colon weight/length) ratios, and ratings for macroscopic and microscopic colon damage. The levels of inflammatory cytokines (interferon-γ and interleukin-4), oxidative stress marker malondialdehyde, and glutathione peroxidase in serum samples were evaluated. Foxp3 T-reg expression was carried out in colonic and splenic tissues. Results: T. spiralis larvae E/S Ags/Ca-BTC MOFs were the most effective in alleviating severe inflammation in murine colitis. The survival rate, disease activity index score, colon weight/length and colon weight/bodyweight ratios, and gross and microscopic colon damage scores have all considerably improved. A large decrease in proinflammatory cytokine (interferon-γ) and oxidative stress marker (malondialdehyde) expression and a significant increase in interleukin-4 and glutathione peroxidase expression were obtained. The expression of Foxp3+ Treg cells was elevated in colonic and splenic tissues. Conclusion: T. spiralis larvae E/S Ags/Ca-BTC MOFs had the highest anti-inflammatory, antioxidant, and cytoprotective capabilities against murine colitis and might be used to develop new preventative and treatment strategies. |
