A novel haemodilution chip mounted on the total thrombus-formation analysis system, a flow chamber system, enables stable analysis of platelet products under low platelet concentration/high shear conditions.

Autor: Fuchizaki A; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Yasui K; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Hayashi T; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Tanaka M; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Oyamada C; Fujimori Kogyo Kabushiki Kaisha Kenkyujo, Yokohama, Japan., Ohnishi-Wada T; Fujimori Kogyo Kabushiki Kaisha Kenkyujo, Yokohama, Japan., Hosokawa K; Fujimori Kogyo Kabushiki Kaisha Kenkyujo, Yokohama, Japan., Fujimura Y; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Shimogaki K; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Hirayama F; Japanese Red Cross Osaka Blood Center, Osaka, Japan., Kimura T; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan., Takihara Y; Japanese Red Cross Kinki Block Blood Center, Ibaraki, Japan.
Jazyk: angličtina
Zdroj: Vox sanguinis [Vox Sang] 2024 Aug; Vol. 119 (8), pp. 883-887. Date of Electronic Publication: 2024 May 24.
DOI: 10.1111/vox.13683
Abstrakt: Background and Objectives: The total thrombus-formation analysis system (T-TAS) can quantitatively analyse the contribution of platelets to haemostasis using reconstituted blood samples. However, it is unsuitable in cases with low platelet counts. We introduced a haemodilution (HD) chip with a shallow chamber depth, adapted to low platelet counts and high shear conditions (1500 s -1 ).
Materials and Methods: Blood samples were prepared by mixing red blood cell products, standard human plasma and platelet products; the final platelet count was 50 × 10 3 /μL. Aggregation tests were performed by using the aggregation inducers collagen, adenosine diphosphate (ADP) and ristocetin. Samples with 2-, 4- and 9-day-old platelet products (N = 10) were evaluated.
Results: The HD chip enabled the stable analysis of the haemostatic function of all samples at a platelet count of 50 × 10 3 /μL. Haemostatic function was correlated with ADP aggregation (time to 10 kPa [T 10 ]: r = -0.53; area under the curve for 30 min: r = 0.40) and storage period (T 10 : r = 0.44).
Conclusion: The HD chip-mounted T-TAS can stably analyse haemostatic function under low platelet counts and high shear conditions; this approach is expected to serve as a bridge to in vivo haemostatic tests with experimental animals.
(© 2024 International Society of Blood Transfusion.)
Databáze: MEDLINE
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