Mechanical characterization of regenerating Hydra tissue spheres.

Autor:	Perros T; University Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, Villeurbanne, France., Biquet-Bisquert A; University Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, Villeurbanne, France; Centre de Biologie Structurale, CNRS UMR5048, INSERM U1054, University of Montpellier, Montpellier, France., Ben Meriem Z; Laboratory for Analysis and Architecture of Systems, Université de Toulouse-CNRS, Toulouse, France., Delarue M; Laboratory for Analysis and Architecture of Systems, Université de Toulouse-CNRS, Toulouse, France., Joseph P; Laboratory for Analysis and Architecture of Systems, Université de Toulouse-CNRS, Toulouse, France., Marcq P; Laboratoire Physique et Mécanique des Milieux Hétérogènes, Sorbonne Université, CNRS UMR 7636, ESPCI, Université Paris Cité, Paris, France., Cochet-Escartin O; University Claude Bernard Lyon 1, CNRS, Institut Lumière Matière, Villeurbanne, France. Electronic address: olivier.cochet-escartin@univ-lyon1.fr.
Jazyk:	angličtina
Zdroj:	Biophysical journal [Biophys J] 2024 Jul 02; Vol. 123 (13), pp. 1792-1803. Date of Electronic Publication: 2024 May 23.
DOI:	10.1016/j.bpj.2024.05.022
Abstrakt:	Hydra vulgaris, long known for its remarkable regenerative capabilities, is also a long-standing source of inspiration for models of spontaneous patterning. Recently it became clear that early patterning during Hydra regeneration is an integrated mechanochemical process whereby morphogen dynamics is influenced by tissue mechanics. One roadblock to understanding Hydra self-organization is our lack of knowledge about the mechanical properties of these organisms. In this study, we combined microfluidic developments to perform parallelized microaspiration rheological experiments and numerical simulations to characterize these mechanical properties. We found three different behaviors depending on the applied stresses: an elastic response, a viscoelastic response, and tissue rupture. Using models of deformable shells, we quantify their Young's modulus, shear viscosity, and the critical stresses required to switch between behaviors. Based on these experimental results, we propose a description of the tissue mechanics during normal regeneration. Our results provide a first step toward the development of original mechanochemical models of patterning grounded in quantitative experimental data. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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