A 3D in vitro model for assessing the influence of intraocular lens: Posterior lens capsule interactions on lens epithelial cell responses.

Autor: Izuagbe S; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Roy J; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Chatila A; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Hoang LQ; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Ea V; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Vaish B; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Co CM; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Ly A; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA., Wu H; Department of Pharmaceutical Sciences, College of Pharmacy, North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, TX, 76107, USA., Tang L; Department of Bioengineering, University of Texas at Arlington, Arlington, TX, 76019, USA. Electronic address: ltang@uta.edu.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2024 Jul; Vol. 244, pp. 109940. Date of Electronic Publication: 2024 May 22.
DOI: 10.1016/j.exer.2024.109940
Abstrakt: Posterior Capsule Opacification (PCO), the most frequent complication of cataract surgery, is caused by the infiltration and proliferation of lens epithelial cells (LECs) at the interface between the intraocular lens (IOL) and posterior lens capsule (PLC). According to the "no space, no cells, no PCO" theory, high affinity (or adhesion force) between the IOL and PLC would decrease the IOL: PLC interface space, hinder LEC migration, and thus reduce PCO formation. To test this hypothesis, an in vitro hemisphere-shaped simulated PLC (sPLC) was made to mimic the human IOL: PLC physical interactions and to assess their influence on LEC responses. Three commercially available IOLs with different affinities/adhesion forces toward the sPLC, including Acrylic foldable IOL, Silicone IOL, and PMMA IOL, were used in this investigation. Using the system, the physical interactions between IOLs and sPLC were quantified by measuring the adhesion force and interface space using an adhesion force apparatus and Optical Coherence Tomography, respectively. Our data shows that high adhesion force and tight binding between IOL and sPLC contribute to a small interface space (or "no space"). By introducing LECs into the in vitro system, we found that, with small interface space, among all IOLs, acrylic foldable IOLs permitted the least extent of LEC infiltration, proliferation, and differentiation (or "no cells"). Further statistical analyses using clinical data revealed that weak LEC responses are associated with low clinical PCO incidence rates (or "no PCO"). The findings support that the in vitro system could simulate IOL: PLC interplays and predict IOLs' PCO potential in support of the "no space, no cells, no PCO" hypothesis.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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