Role of brain monoamines in acetamiprid-induced anxiety-like behavior.

Autor: Hirai A; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan., Toda C; Department of Neuroscience for Metabolic Control, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, Japan., Yohannes YB; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan., Collins N; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan., Tamba M; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan., Nomiyama K; Center for Marine Environmental Studies, Division of Environmental Chemistry and Ecotoxicology, Ehime University, Matsuyama 790-8577, Japan., Eguchi A; Department of Sustainable Health Science, Center for Preventive Medical Sciences, Chiba University, Chiba 263-8522, Japan., Hoshi N; Department of Animal Science, Laboratory of Animal Molecular Morphology, Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan., Hirano T; Faculty of Pharmaceutical Sciences, University of Toyama, Toyama 930-0194, Japan., Nakayama SMM; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan; School of Veterinary Medicine, The University of Zambia, Lusaka P.O. Box 32379, Zambia., Ishizuka M; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan., Ikenaka Y; Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan; Translational Research Unit, Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan; One Health Research Center, Hokkaido University, Sapporo 060-0818, Japan; Water Research Group, Unit for Environmental Sciences and Management, North-West University, Potchefstroom, South Africa. Electronic address: y_ikenaka@vetmed.hokudai.ac.jp.
Jazyk: angličtina
Zdroj: Toxicology [Toxicology] 2024 Jun; Vol. 505, pp. 153839. Date of Electronic Publication: 2024 May 21.
DOI: 10.1016/j.tox.2024.153839
Abstrakt: Neonicotinoid (NN) pesticides have been linked to increased brain dysfunction in mammals, such as anxiety-like behavior; this is thought to involve monoamines (MA), neurotransmitters that control behavior, memory, and learning. However, the mechanism by which NNs affect the central nervous system is not fully understood. In this study, we aimed to investigate whether MAs affect NNs-induced anxiety-like behavior. Mice were orally administered acetamiprid (ACE), an NN, at the no observed adverse effect level (NOAEL) of mouse (20 mg/kg body mass) set by the Food Safety Commission of Japan, and the elevated zero-maze (EZM) test was performed 30 min after administration. After behavioral analysis, levels of four MA (dopamine, 3-MT, serotonin, and histamine) in selected brain regions were determined by liquid chromatography mass spectrometry (LC/MS/MS). In the exposed group, a trend toward increased anxiety-like behavior was observed, and at least one MA concentration was significantly increased in each region. Further, significant correlations were found between behavioral test results and hippocampal serotonin and striatal dopamine concentrations, as well as between dopamine and serotonin concentrations, in the exposed group. As anxiety can influence activity in the behavioral tests, the activity of neurons in the raphe nuclei (RN), a brain region greatly involved in anxiety via the serotonergic system, was examined by staining with anti-serotonin antibodies, and increased serotonergic activity was observed. Taken together, these results suggest that ACE regulates MA levels, notably serotonin levels in the hippocampus and that RN plays an important role in ACE-induced anxiety-like behavior.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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