The basic functions of rabbit ovarian granulosa cell are regulated by adipokines monocyte chemoattractant protein-1 and plasminogen activator inhibitor-1.

Autor: Sirotkin AV; Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovakia. Electronic address: asirotkin@ukf.sk., Fabová Z; Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovakia., Loncová B; Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Nitra, Slovakia., Harrath AH; College of Science, King Saud University, Riyadh, Saudi Arabia.
Jazyk: angličtina
Zdroj: Domestic animal endocrinology [Domest Anim Endocrinol] 2024 Jul; Vol. 88, pp. 106856. Date of Electronic Publication: 2024 May 09.
DOI: 10.1016/j.domaniend.2024.106856
Abstrakt: The aim of the present study was to examine the influence of monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1) on ovarian cell functions. Rabbit ovarian granulosa cells were cultured with or without MCP-1 or PAI-1 (at 0, 0.1, 1, or 10 ng/ml). Cell viability, proliferation, cytoplasmic apoptosis and release of progesterone and estradiol were measured by Cell Counting Kit-8 (CCK-8), BrdU incorporation, and cell death detection assays and ELISA. The addition of either MCP-1 or PAI-1 increased cell viability and proliferation and decreased apoptosis. MCP-1 promoted, while PAI-1 suppressed, progesterone release. Both MCP-1 and PAI-1 reduced estradiol output. The present results suggest that MCP-1 or PAI-1 can be physiological promoters of rabbit ovarian cell viability and proliferation, inhibitors of apoptosis and regulators of ovarian steroidogenesis.
Competing Interests: Declaration of competing interest The authors have no conflicts of interest to declare.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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