Fecal microbiota and volatile metabolome pattern alterations precede late-onset meningitis in preterm neonates.

Autor:	Frerichs NM; Amsterdam UMC, University of Amsterdam, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Department of Pediatric Gastroenterology, Emma Children's Hospital.; Amsterdam UMC, University of Amsterdam, Amsterdam Reproduction and Development Research Institute, Amsterdam, 1105 AZ, The Netherlands., Deianova N; Amsterdam UMC, University of Amsterdam, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Department of Pediatric Gastroenterology, Emma Children's Hospital.; Amsterdam UMC, University of Amsterdam, Amsterdam Reproduction and Development Research Institute, Amsterdam, 1105 AZ, The Netherlands., El Manouni El Hassani S; Amsterdam UMC, University of Amsterdam, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Department of Pediatric Gastroenterology, Emma Children's Hospital.; Amsterdam UMC, University of Amsterdam, Amsterdam Reproduction and Development Research Institute, Amsterdam, 1105 AZ, The Netherlands., Acharjee A; Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK.; Institute of Translational Medicine, University Hospitals Birmingham NHS Foundation Trust, Birmingham, B15 2TT, UK.; Health Data Research UK (HDR UK), London, NW1 2BE, UK., Quraishi MN; Institute of Cancer and Genomic Sciences, University of Birmingham, Birmingham, B15 2TT, UK., de Boode WP; Neonatal Intensive Care Unit, Radboud University Medical Centre, Radboud Institute for Health Sciences, Amalia Children's Hospital, Nijmegen, 6525 GA, The Netherlands., Cossey V; Neonatal Intensive Care Unit, University Hospitals Leuven, Leuven, 3000, Belgium., Hulzebos CV; Neonatal Intensive Care Unit, Beatrix Children's Hospital/University Medical Centre Groningen, Groningen, 9713 GZ, The Netherlands., van Kaam AH; Department of Neonatology, Emma Children's Hospital; Amsterdam Reproduction and Development Research Institute, Amsterdam, 1105 AZ, the Netherlands., Kramer BW; University of Western Australia, Crawley, Australia; Neuroplast BV, Maastricht, 6167 RD, The Netherlands., d'Haens E; Neonatal Intensive Care Unit, Amalia Children's Centre, Isala, Zwolle, 8025 AB, The Netherlands., de Jonge WJ; Tytgat Institute for Liver and Intestinal Research, Amsterdam UMC, Location AMC, Amsterdam, 1105 AZ, The Netherlands.; Department of Surgery, University of Bonn, Bonn, D-53113, Germany., Vijlbrief DC; Neonatal Intensive Care Unit, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, 3584 CX, The Netherlands., van Weissenbruch MM; Department of Neonatology, Emma Children's Hospital; Amsterdam Reproduction and Development Research Institute, Amsterdam, 1105 AZ, the Netherlands., Daulton E; School of Engineering, University of Warwick, Coventry, CV4 7AL, United Kingdom., Wicaksono AN; School of Engineering, University of Warwick, Coventry, CV4 7AL, United Kingdom., Covington JA; School of Engineering, University of Warwick, Coventry, CV4 7AL, United Kingdom., Benninga MA; Amsterdam UMC, University of Amsterdam, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Department of Pediatric Gastroenterology, Emma Children's Hospital., de Boer NKH; Department of Gastroenterology and Hepatology, Amsterdam UMC, Vrije Universiteit, AGEM Research Institute, Amsterdam, 1105 AZ, The Netherlands., van Goudoever JB; Amsterdam UMC, University of Amsterdam, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Department of Pediatric Gastroenterology, Emma Children's Hospital., Niemarkt HJ; Neonatal Intensive Care Unit, Máxima Medical Centre, Veldhoven, 5504 DB, The Netherlands., de Meij TGJ; Amsterdam UMC, University of Amsterdam, Amsterdam Gastroenterology Endocrinology Metabolism Research Institute, Department of Pediatric Gastroenterology, Emma Children's Hospital.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2024 May 23. Date of Electronic Publication: 2024 May 23.
DOI:	10.1093/infdis/jiae265
Abstrakt:	Objective: The fecal microbiota and metabolome are hypothesized to be altered before late-onset neonatal meningitis (LOM), in analogy to late-onset sepsis (LOS). The present study aimed to identify fecal microbiota composition and volatile metabolomics preceding LOM. Methods: Cases and gestational age-matched controls were selected from a prospective, longitudinal preterm cohort study (born <30 weeks' gestation) at nine neonatal intensive care units. The microbial composition (16S rRNA sequencing) and volatile metabolome (gas chromatography-ion mobility spectrometry (GC-IMS) and GC-time-of-flight-mass spectrometry (GC-TOF-MS)), were analyzed in fecal samples 1-10 days pre-LOM. Results: Of 1397 included infants, 21 were diagnosed with LOM (1.5%), and 19 with concomitant LOS (90%). Random Forest classification and MaAsLin2 analysis found similar microbiota features contribute to the discrimination of fecal pre-LOM samples versus controls. A Random Forest model based on six microbiota features accurately predicts LOM 1-3 days before diagnosis with an area under the curve (AUC) of 0.88 (n=147). Pattern recognition analysis by GC-IMS revealed an AUC of 0.70-0.76 (P<0.05) in the three days pre-LOM (n=92). No single discriminative metabolites were identified by GC-TOF-MS (n=66). Conclusion: Infants with LOM could be accurately discriminated from controls based on preclinical microbiota composition, while alterations in the volatile metabolome were moderately associated with preclinical LOM. (© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America.)
Databáze:	MEDLINE
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