In-Brain Multiphoton Imaging of Vaterite Cargoes Loaded with Carbon Dots.

Autor:	Barhum H; Department of Electrical Engineering, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.; Triangle Regional Research and Development Center, Kfar Qara 3007500, Israel.; Light-Matter Interaction Centre, Tel Aviv University, Tel Aviv 69978, Israel., McDonnell C; Department of Electrical Engineering, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.; Light-Matter Interaction Centre, Tel Aviv University, Tel Aviv 69978, Israel., Peltek O; School of Physics and Engineering, ITMO University, St. Petersburg 191002, Russian Federation., Jain R; Neurobiology, Biochemistry and Biophysics School, Wise Life Science Faculty, Tel Aviv University, Tel Aviv 69978, Israel., Amer M; Triangle Regional Research and Development Center, Kfar Qara 3007500, Israel., Kain D; Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 69978, Israel., Elad-Sfadia G; Neurobiology, Biochemistry and Biophysics School, Wise Life Science Faculty, Tel Aviv University, Tel Aviv 69978, Israel., Athamna M; Triangle Regional Research and Development Center, Kfar Qara 3007500, Israel.; Neurobiology, Biochemistry and Biophysics School, Wise Life Science Faculty, Tel Aviv University, Tel Aviv 69978, Israel., Blinder P; Neurobiology, Biochemistry and Biophysics School, Wise Life Science Faculty, Tel Aviv University, Tel Aviv 69978, Israel.; Sagol School of Neuroscience, Tel Aviv University, Tel Aviv 69978, Israel., Ginzburg P; Department of Electrical Engineering, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel.; Light-Matter Interaction Centre, Tel Aviv University, Tel Aviv 69978, Israel.
Jazyk:	angličtina
Zdroj:	Nano letters [Nano Lett] 2024 Jul 10; Vol. 24 (27), pp. 8232-8239. Date of Electronic Publication: 2024 May 23.
DOI:	10.1021/acs.nanolett.4c00325
Abstrakt:	Biocompatible fluorescent agents are key contributors to the theranostic paradigm by enabling real-time in vivo imaging. This study explores the optical properties of phenylenediamine carbon dots (CDs) and demonstrates their potential for fluorescence imaging in cells and brain blood vessels. The nonlinear absorption cross-section of the CDs was measured and achieved values near 50 Goeppert-Mayer (GM) units with efficient excitation in the 775-895 nm spectral range. Mesoporous vaterite nanoparticles were loaded with CDs to examine the possibility of a biocompatible imaging platform. Efficient one- and two-photon imaging of the CD-vaterite composites uptaken by diverse cells was demonstrated. For an in vivo scenario, CD-vaterite composites were injected into the bloodstream of a mouse, and their flow was monitored within the blood vessels of the brain through a cranial window. These results show the potential of the platform for high-brightness biocompatible imaging with the potential for both sensing and simultaneous drug delivery.
Databáze:	MEDLINE
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