Can Mitotic Figures in Hyperchromatic Crowded Groups be Cytodiagnostic Criteria for High-Grade Squamous Intra-epithelial Lesions?

Autor:	Suzuki H; Department of Pathology, Nara Medical University Hospital, Nara, Japan., Kondo Y; Department of Medical Technology, The Graduate School of Ehime Prefectural University of Health Sciences, Ehime, Japan., Oda C; Department of Medical Technology, The Graduate School of Ehime Prefectural University of Health Sciences, Ehime, Japan., Nishikawa T; Department of Pathology, Nara Medical University Hospital, Nara, Japan., Takeuchi M; Department of Pathology, Nara Medical University Hospital, Nara, Japan., Tatsumi S; Department of Pathology, Nara Medical University Hospital, Nara, Japan., Hosokawa S; Department of Medical Technology, The Graduate School of Ehime Prefectural University of Health Sciences, Ehime, Japan.; Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Ehime, Japan., Irino S; Department of Nursing, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Ehime, Japan., Uchiyama T; Department of Diagnostic Pathology, Nara Medical University, Nara, Japan., Fujii T; Department of Diagnostic Pathology, Nara Medical University, Nara, Japan., Norimatsu Y; Department of Medical Technology, The Graduate School of Ehime Prefectural University of Health Sciences, Ehime, Japan.; Department of Medical Technology, Faculty of Health Sciences, Ehime Prefectural University of Health Sciences, Ehime, Japan.
Jazyk:	angličtina
Zdroj:	Journal of cytology [J Cytol] 2024 Apr-Jun; Vol. 41 (2), pp. 116-122. Date of Electronic Publication: 2024 Apr 24.
DOI:	10.4103/joc.joc_156_23
Abstrakt:	Aims: The present study aimed to investigate whether the presence of mitoses in hyperchromatic crowded groups (HCGs) in cervical cytological specimens can serve as cytological criteria for high-grade squamous intra-epithelial lesions (HSILs). Methods and Material: Various parameters were examined, including the frequency of mitotic figures per high power field (HPF) in Pap, hematoxylin eosin (HE) samples, and PHH3 immunocytochemical (ICC) and immunohistochemical (IHC) analyses. Results: In the Pap and PHH3-ICC samples, the number of mitotic figures observed in HCGs was significantly higher in HSIL ( P < 0.001) compared to other groups. Furthermore, the frequency of observing two or more mitoses was significantly higher in HSIL (Pap: P = 0.002, PHH3-ICC: P < 0.001) than in low-grade squamous intra-epithelial lesions (LSILs). Moreover, a comparison between Pap samples and PHH3-ICC showed that the frequency of two or more mitoses was significantly higher in the PHH3-ICC analysis of HSIL ( P = 0.042). Regarding HE and PHH3-IHC samples, counting the number of mitoses in the lower and middle/upper layers of the squamous epithelial layer revealed that HSIL had a significantly higher value (HE: P = 0.0089, PHH3-IHC: P = 0.0002) than LSIL in the middle/upper layers. Conclusions: Hence, the presence of two or more mitotic figures in HCGs per HPF in cervical cytology indicates a suspicion of HSIL. The detection of mitoses in PHH3-ICC samples is more sensitive and easier to observe than in Pap samples, making it a valuable mitotic marker. Competing Interests: There are no conflicts of interest. (Copyright: © 2024 Journal of Cytology.)
Databáze:	MEDLINE
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