Bacterial community of ticks (Acari: Ixodidae) and mammals from Arauca, Colombian Orinoquia.

Autor:	Ossa-López PA; Doctorado en Ciencias, Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia.; Grupo de Investigación en Genética, Biodiversidad y Manejo de Ecosistemas (GEBIOME), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia., Ramírez-Chaves HE; Grupo de Investigación en Genética, Biodiversidad y Manejo de Ecosistemas (GEBIOME), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia.; Centro de Museos, Museo de Historia Natural, Universidad de Caldas, Calle 58 No. 21-50, 170004, Manizales, Caldas, Colombia., Álvarez López ME; Grupo de Investigación en Genética, Biodiversidad y Manejo de Ecosistemas (GEBIOME), Departamento de Ciencias Básicas, Facultad de Ciencias para la Salud, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia., Castaño Villa GJ; Grupo de Investigación en Genética, Biodiversidad y Manejo de Ecosistemas (GEBIOME), Departamento de Desarrollo Rural y Recursos Naturales, Facultad de Ciencias Agropecuarias, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia., Rivera-Páez FA; Grupo de Investigación en Genética, Biodiversidad y Manejo de Ecosistemas (GEBIOME), Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10, 170004, Manizales, Caldas, Colombia.
Jazyk:	angličtina
Zdroj:	International journal for parasitology. Parasites and wildlife [Int J Parasitol Parasites Wildl] 2024 May 09; Vol. 24, pp. 100943. Date of Electronic Publication: 2024 May 09 (Print Publication: 2024).
DOI:	10.1016/j.ijppaw.2024.100943
Abstrakt:	Ticks are obligate hematophagous ectoparasites of vertebrates and are relevant worldwide due to the number of bacterial and other pathogens they can transmit. To date, the knowledge about the microorganisms that ticks harbor and transmit to their hosts is incipient. In this study, 24 samples of mammals belonging to four taxonomic orders and ticks of the genera Amblyomma and Rhipicephalus from the Orinoco region of Colombia were analyzed to described and compare the bacterial microbiome. Genetic extraction was performed, and the V3-V4 region of the 16S rRNA gene was amplified by PCR. Libraries were created, and those samples with adequate quality indices were sequenced using Illumina MiSeq technology. Bacterial taxonomic assignment analyses were conducted through Amplicon Sequence Variants (ASVs) and Operational Taxonomic Units (OTUs). The results correspond to 16 samples that passed the quality filters, with 3218 OTUs (415 families). Although a considerable number of unknown bacteria was found, Enterobacteriaceae, Beijerinckiaceae, Moraxellaceae, and Burkholderiaceae are the most prevalent families, and the presence of the genera Coxiella , Escherichia-Shigella , Enterobacter , which can harbor pathogenic species was confirmed. In individuals of Amblyomma mixtum found actively feeding on Hydrochoerus hydrochaeris , bacteria of the genera Escherichia-Shigella and Enterobacter were documented. Similarly, Rhipicephalus microplus found actively feeding on Odocoileus virginianus cariacou shared Escherichia-Shigella . Ralstonia was shared among the blood samples of H. hydrochaeris , while Anaplasma and Eubacterium were shared in blood and liver samples of O. v. cariacou . Shared bacteria between A. mixtum and R. microplus included Bacillus , Coxiella , and Escherichia-Shigella . The results highlight the need of additional studies in other natural regions of Colombia and other American countries where tick-borne diseases have been detected. Likewise, the recorded data are the first at the level of bacterial communities in ticks of the family Ixodidae and provide valuable knowledge for the understanding host-tick and pathogen interactions. Competing Interests: The authors disclose that no substantial portion of the study has been published or is under consideration for publication elsewhere and that its submission for publication has been approved by all the authors. All authors approve the submission of this article to International Journal for Parasitology: Parasites and Wildlife. The authors have no conflicts of interest to declare. (© 2024 The Authors.)
Databáze:	MEDLINE
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