Osmosensor-mediated control of Ca 2+ spiking in pollen germination.

Autor: Pei S; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China.; Department of Biology, Duke University, Durham, NC, USA.; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA.; College of Life Sciences, Zhejiang University, Hangzhou, China.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Tao Q; College of Life Sciences, Zhejiang University, Hangzhou, China.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Li W; College of Life Sciences, Zhejiang University, Hangzhou, China.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Qi G; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Wang B; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Wang Y; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China., Dai S; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China., Shen Q; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Wang X; College of Life Sciences, Zhejiang University, Hangzhou, China.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Wu X; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Xu S; Department of Biology, Duke University, Durham, NC, USA., Theprungsirikul L; Department of Biology, Duke University, Durham, NC, USA., Zhang J; Department of Biology, Duke University, Durham, NC, USA., Liang L; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Liu Y; College of Life Sciences, Zhejiang University, Hangzhou, China.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Chen K; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Shen Y; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Crawford BM; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA., Cheng M; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Zhang Q; College of Life Sciences, Zhejiang University, Hangzhou, China.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Wang Y; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Liu H; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Yang B; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China., Krichilsky B; Department of Biology, Duke University, Durham, NC, USA., Pei J; Department of Biology, Duke University, Durham, NC, USA.; Fuqua School of Business, Duke University, Durham, NC, USA., Song K; Department of Biology, Duke University, Durham, NC, USA., Johnson DM; Department of Physics, Duke University, Durham, NC, USA., Jiang Z; Department of Biology, Duke University, Durham, NC, USA., Wu F; Department of Biology, Duke University, Durham, NC, USA., Swift GB; Department of Physics, Duke University, Durham, NC, USA., Yang H; Department of Biochemistry, Duke University, Durham, NC, USA., Liu Z; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China., Zou X; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China., Vo-Dinh T; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA., Liu F; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China. jwszjx@hunau.edu.cn.; Department of Biology, Duke University, Durham, NC, USA. jwszjx@hunau.edu.cn., Pei ZM; Department of Biology, Duke University, Durham, NC, USA. zpei@duke.edu.; Fitzpatrick Institute for Photonics, Duke University, Durham, NC, USA. zpei@duke.edu., Yuan F; College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China. yuanfang800@yahoo.com.; Department of Biology, Duke University, Durham, NC, USA. yuanfang800@yahoo.com.; College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China. yuanfang800@yahoo.com.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 May; Vol. 629 (8014), pp. 1118-1125. Date of Electronic Publication: 2024 May 22.
DOI: 10.1038/s41586-024-07445-6
Abstrakt: Higher plants survive terrestrial water deficiency and fluctuation by arresting cellular activities (dehydration) and resuscitating processes (rehydration). However, how plants monitor water availability during rehydration is unknown. Although increases in hypo-osmolarity-induced cytosolic Ca 2+ concentration (HOSCA) have long been postulated to be the mechanism for sensing hypo-osmolarity in rehydration 1,2 , the molecular basis remains unknown. Because osmolarity triggers membrane tension and the osmosensing specificity of osmosensing channels can only be determined in vivo 3-5 , these channels have been classified as a subtype of mechanosensors. Here we identify bona fide cell surface hypo-osmosensors in Arabidopsis and find that pollen Ca 2+ spiking is controlled directly by water through these hypo-osmosensors-that is, Ca 2+ spiking is the second messenger for water status. We developed a functional expression screen in Escherichia coli for hypo-osmosensitive channels and identified OSCA2.1, a member of the hyperosmolarity-gated calcium-permeable channel (OSCA) family of proteins 6 . We screened single and high-order OSCA mutants, and observed that the osca2.1/osca2.2 double-knockout mutant was impaired in pollen germination and HOSCA. OSCA2.1 and OSCA2.2 function as hypo-osmosensitive Ca 2+ -permeable channels in planta and in HEK293 cells. Decreasing osmolarity of the medium enhanced pollen Ca 2+ oscillations, which were mediated by OSCA2.1 and OSCA2.2 and required for germination. OSCA2.1 and OSCA2.2 convert extracellular water status into Ca 2+ spiking in pollen and may serve as essential hypo-osmosensors for tracking rehydration in plants.
(© 2024. The Author(s).)
Databáze: MEDLINE
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