Prolonged Activity Deprivation Causes Pre- and Postsynaptic Compensatory Plasticity at Neocortical Excitatory Synapses.

Autor:	Wise DL; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Escobedo-Lozoya Y; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Valakh V; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Gao EY; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Bhonsle A; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Lei QL; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Cheng X; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Greene SB; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Van Hooser SD; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110., Nelson SB; Department of Biology, Brandeis University, Waltham, Massachusetts 02454 9110 nelson@brandeis.edu.
Jazyk:	angličtina
Zdroj:	ENeuro [eNeuro] 2024 Jun 07; Vol. 11 (6). Date of Electronic Publication: 2024 Jun 07 (Print Publication: 2024).
DOI:	10.1523/ENEURO.0366-23.2024
Abstrakt:	Homeostatic plasticity stabilizes firing rates of neurons, but the pressure to restore low activity rates can significantly alter synaptic and cellular properties. Most previous studies of homeostatic readjustment to complete activity silencing in rodent forebrain have examined changes after 2 d of deprivation, but it is known that longer periods of deprivation can produce adverse effects. To better understand the mechanisms underlying these effects and to address how presynaptic as well as postsynaptic compartments change during homeostatic plasticity, we subjected mouse cortical slice cultures to a more severe 5 d deprivation paradigm. We developed and validated a computational framework to measure the number and morphology of presynaptic and postsynaptic compartments from super-resolution light microscopy images of dense cortical tissue. Using these tools, combined with electrophysiological miniature excitatory postsynaptic current measurements, and synaptic imaging at the electron microscopy level, we assessed the functional and morphological results of prolonged deprivation. Excitatory synapses were strengthened both presynaptically and postsynaptically. Surprisingly, we also observed a decrement in the density of excitatory synapses, both as measured from colocalized staining of pre- and postsynaptic proteins in tissue and from the number of dendritic spines. Overall, our results suggest that cortical networks deprived of activity progressively move toward a smaller population of stronger synapses. Competing Interests: The authors declare no competing financial interests. (Copyright © 2024 Wise et al.)
Databáze:	MEDLINE
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