The tissue-specific nature of physiological zebrafish mitochondrial bioenergetics.

Autor: Azevedo RDS; Laboratório de Biologia Celular e Molecular, Universidade de Pernambuco - UPE, Campus Garanhuns, Garanhuns, PE, Brazil. Electronic address: rafael.david@upe.br., Falcão KVG; Departamento de Bioquímica, Universidade Federal de Pernambuco, Cidade Universitária, Recife, PE, Brazil., Almeida SMV; Laboratório de Biologia Celular e Molecular, Universidade de Pernambuco - UPE, Campus Garanhuns, Garanhuns, PE, Brazil., Araújo MC; Departamento de Bioquímica, Universidade Federal de Pernambuco, Cidade Universitária, Recife, PE, Brazil., Silva-Filho RC; Departamento de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, Brazil., Souza Maia MB; Departamento de Farmacologia, Universidade Federal de Pernambuco, Recife, PE, Brazil., Amaral IPGD; Centro de Biotecnologia, Universidade Federal de Paraíba, João Pessoa, PB, Brazil., Leite ACR; Departamento de Química e Biotecnologia, Universidade Federal de Alagoas, Maceió, AL, Brazil., de Souza Bezerra R; Departamento de Bioquímica, Universidade Federal de Pernambuco, Cidade Universitária, Recife, PE, Brazil.
Jazyk: angličtina
Zdroj: Mitochondrion [Mitochondrion] 2024 Jul; Vol. 77, pp. 101901. Date of Electronic Publication: 2024 May 21.
DOI: 10.1016/j.mito.2024.101901
Abstrakt: Zebrafish are a powerful tool to study a myriad of experimental conditions, including mitochondrial bioenergetics. Considering that mitochondria are different in many aspects depending on the tissue evaluated, in the zebrafish model there is still a lack of this investigation. Especially for juvenile zebrafish. In the present study, we examined whether different tissues from zebrafish juveniles show mitochondrial density- and tissue-specificity comparing brain, liver, heart, and skeletal muscle (SM). The liver and brain complex IV showed the highest O 2 consumption of all ETC in all tissues (10x when compared to other respiratory complexes). The liver showed a higher potential for ROS generation. In this way, the brain and liver showed more susceptibility to O 2 - generation when compared to other tissues. Regarding Ca 2+ transport, the brain showed greater capacity for Ca 2+ uptake and the liver presented low Ca 2+ uptake capacity. The liver and brain were more susceptible to producing NO. The enzymes SOD and Catalase showed high activity in the brain, whereas GPx showed higher activity in the liver and CS in the SM. TEM reveals, as expected, a physiological diverse mitochondrial morphology. The essential differences between zebrafish tissues investigated probably reflect how the mitochondria play a diverse role in systemic homeostasis. This feature may not be limited to normal metabolic functions but also to stress conditions. In summary, mitochondrial bioenergetics in zebrafish juvenile permeabilized tissues showed a tissue-specificity and a useful tool to investigate conditions of redox system imbalance, mainly in the liver and brain.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. and Mitochondria Research Society. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: