AMPK inhibits voltage-gated calcium channel-current in rat chromaffin cells.

Autor: Fukumoto-Inukai AK; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico., Bermeo K; Licenciatura en Neurociencias, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico., Arenas I; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico., Rosendo-Pineda MJ; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico., Pimentel-Cabrera JA; Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico., Garcia DE; Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México, UNAM, Circuito Exterior S/N, Ciudad Universitaria, Coyoacán, 04510, Ciudad de México, Mexico. Electronic address: erasmo@unam.mx.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 Sep 15; Vol. 591, pp. 112275. Date of Electronic Publication: 2024 May 20.
DOI: 10.1016/j.mce.2024.112275
Abstrakt: Metabolic changes are critical in the regulation of Ca 2+ influx in central and peripheral neuroendocrine cells. To study the regulation of L-type Ca 2+ channels by AMPK we used biochemical reagents and ATP/glucose-concentration manipulations in rat chromaffin cells. AICAR and Compound-C, at low concentration, significantly induce changes in L-type Ca 2+ channel-current amplitude and voltage dependence. Remarkably, an overlasting decrease in the channel-current density can be induced by lowering the intracellular level of ATP. Accordingly, Ca 2+ channel-current density gradually diminishes by decreasing the extracellular glucose concentration. By using immunofluorescence, a decrease in the expression of Ca V 1.2 is observed while decreasing extracellular glucose, suggesting that AMPK reduces the number of functional Ca 2+ channels into the plasma membrane. Together, these results support for the first time the dependence of metabolic changes in the maintenance of Ca 2+ channel-current by AMPK. They reveal a key step in Ca 2+ influx in secretory cells.
Competing Interests: Declaration of competing interest No conflicts of interest, financial or otherwise, are declared by the authors.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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