Red-complex bacteria exhibit distinctly different interactions with human periodontal ligament stromal cells compared to Fusobacterium nucleatum.

Autor: Kendlbacher FL; NanoGlycobiology Research Group, Institute of Biochemistry, Department of Chemistry, Universität für Bodenkultur Wien, Vienna, Austria., Bloch S; NanoGlycobiology Research Group, Institute of Biochemistry, Department of Chemistry, Universität für Bodenkultur Wien, Vienna, Austria., Hager-Mair FF; NanoGlycobiology Research Group, Institute of Biochemistry, Department of Chemistry, Universität für Bodenkultur Wien, Vienna, Austria., Schäffer C; NanoGlycobiology Research Group, Institute of Biochemistry, Department of Chemistry, Universität für Bodenkultur Wien, Vienna, Austria. Electronic address: christina.schaeffer@boku.ac.at., Andrukhov O; Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, A-1090 Vienna, Austria. Electronic address: oleh.andrukhov@meduniwien.ac.at.
Jazyk: angličtina
Zdroj: Archives of oral biology [Arch Oral Biol] 2024 Aug; Vol. 164, pp. 106004. Date of Electronic Publication: 2024 May 16.
DOI: 10.1016/j.archoralbio.2024.106004
Abstrakt: Objective: The red-complex bacteria Porphyromonas gingivalis and Tannerella forsythia together with Fusobacterium nucleatum are essential players in periodontitis. This study investigated the bacterial interplay with human periodontal ligament mesenchymal stromal cells (hPDL-MSCs) which act in the acute phase of periodontal infection.
Design: The capability of the bacteria to induce an inflammatory response as well as their viability, cellular adhesion and invasion were analyzed upon mono- and co-infections of hPDL-MSCs to delineate potential synergistic or antagonistic effects. The expression level and concentration of interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1 were measured using qRT-PCR and ELISA. Viability, invasion, and adhesion were determined quantitatively using agar plate culture and qualitatively by confocal microscopy.
Results: Viability of P. gingivalis and T. forsythia but not F. nucleatum was preserved in the presence of hPDL-MSCs, even in an oxygenated environment. F. nucleatum significantly increased the expression and concentration of IL-6, IL-8 and MCP-1 in hPDL-MSCs, while T. forsythia and P. gingivalis caused only a minimal inflammatory response. Co-infections in different combinations had no effect on the inflammatory response. Moreover, P. gingivalis mitigated the increase in cytokine levels elicited by F. nucleatum. Both red-complex bacteria adhered to and invaded hPDL-MSCs in greater numbers than F. nucleatum, with only a minor effect of co-infections.
Conclusions: Oral bacteria of different pathogenicity status interact differently with hPDL-MSCs. The data support P. gingivalis' capability to manipulate the inflammatory host response. Further research is necessary to obtain a comprehensive picture of the role of hPDL-MSCs in more complex oral biofilms.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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