Symplasmic phloem loading and subcellular transport in storage roots are key factors for carbon allocation in cassava.

Autor:	Rüscher D; Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany., Vasina VV; School of Biological Sciences, Washington State University, Pullman, WA 99163, USA., Knoblauch J; School of Biological Sciences, Washington State University, Pullman, WA 99163, USA., Bellin L; Division of Plant Physiology, Department of Biology, University of Kaiserslautern-Landau (RPTU), Erwin-Schrödinger-Str. 22, 67663 Kaiserslautern, Germany., Pommerrenig B; Division of Plant Physiology, Department of Biology, University of Kaiserslautern-Landau (RPTU), Erwin-Schrödinger-Str. 22, 67663 Kaiserslautern, Germany., Alseekh S; Division of Central Metabolism, Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany., Fernie AR; Division of Central Metabolism, Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany., Neuhaus HE; Division of Plant Physiology, Department of Biology, University of Kaiserslautern-Landau (RPTU), Erwin-Schrödinger-Str. 22, 67663 Kaiserslautern, Germany., Knoblauch M; School of Biological Sciences, Washington State University, Pullman, WA 99163, USA., Sonnewald U; Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany., Zierer W; Division of Biochemistry, Department of Biology, Friedrich-Alexander-University Erlangen-Nuremberg, Staudtstrasse 5, 91058 Erlangen, Germany.
Jazyk:	angličtina
Zdroj:	Plant physiology [Plant Physiol] 2024 Oct 01; Vol. 196 (2), pp. 1322-1339.
DOI:	10.1093/plphys/kiae298
Abstrakt:	Cassava (Manihot esculenta) is a deciduous woody perennial shrub that stores large amounts of carbon and water in its storage roots. Previous studies have shown that assimilating unloading into storage roots happens symplasmically once secondary anatomy is established. However, mechanisms controlling phloem loading and overall carbon partitioning to different cassava tissues remain unclear. Here, we used a combination of histological, transcriptional, and biochemical analyses on different cassava tissues and at different timepoints to better understand source-sink carbon allocation. We found that cassava likely utilizes a predominantly passive symplasmic phloem loading strategy, indicated by the lack of expression of genes coding for key players of sucrose transport, the existence of branched plasmodesmata in the companion cell/bundle sheath interface of minor leaf veins, and very high leaf sucrose concentrations. Furthermore, we showed that tissue-specific changes in anatomy and non-structural carbohydrate contents are associated with tissue-specific modification in gene expression for sucrose cleavage/synthesis, as well as subcellular compartmentalization of sugars. Overall, our data suggest that carbon allocation during storage root filling is mostly facilitated symplasmically and is likely mostly regulated by local tissue demand and subcellular compartmentalization. Competing Interests: Conflict of interest statement. The authors have declared that no competing interest exists. (© The Author(s) 2024. Published by Oxford University Press on behalf of American Society of Plant Biologists.)
Databáze:	MEDLINE
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