Leishmania (Sauroleishmania) tarentolae versus pathogenic species: comparative evaluation of protease activity, glycoconjugates, resistance to complement and metabolome composition.

Autor:	Andrade FFD; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Biologia Geral, Belo Horizonte, MG, Brasil., Vitório JG; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Canuto GAB; Universidade Federal da Bahia, Instituto de Química, Departamento de Química Analítica, Salvador, BA, Brasil., Nunes FFC; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Rodrigues IA; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Almeida APMM; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Nascimento FC; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Costa AO; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Vieira TDS; Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Grupo Biotecnologia Aplicada ao Estudo de Patógenos, Belo Horizonte, MG, Brasil., Silva ACC; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Biologia Geral, Belo Horizonte, MG, Brasil., André LC; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Gontijo CMF; Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Grupo Biotecnologia Aplicada ao Estudo de Patógenos, Belo Horizonte, MG, Brasil., Junqueira C; Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Grupo Biotecnologia Aplicada ao Estudo de Patógenos, Belo Horizonte, MG, Brasil., Toledo JS; Universidade Federal de Minas Gerais, Faculdade de Farmácia, Departamento de Análises Clínicas e Toxicológicas, Belo Horizonte, MG, Brasil., Fernandes AP; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Biologia Geral, Belo Horizonte, MG, Brasil., Soares RP; Fundação Oswaldo Cruz-Fiocruz, Instituto René Rachou, Grupo Biotecnologia Aplicada ao Estudo de Patógenos, Belo Horizonte, MG, Brasil.
Jazyk:	angličtina
Zdroj:	Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2024 May 20; Vol. 119, pp. e230243. Date of Electronic Publication: 2024 May 20 (Print Publication: 2024).
DOI:	10.1590/0074-02760230243
Abstrakt:	Background: Leishmania tarentolae is a non-pathogenic species found in lizards representing an important model for Leishmania biology. However, several aspects of this Sauroleishmania remain unknown to explain its low level of virulence. Objectives: We reported several aspects of L. tarentolae biology including glycoconjugates, proteolytic activities and metabolome composition in comparison to pathogenic species (Leishmania amazonensis, Leishmania braziliensis, Leishmania infantum and Leishmania major). Methods: Parasites were cultured for extraction and purification of lipophosphoglycan (LPG), immunofluorescence probing with anti-gp63 and resistance against complement. Parasite extracts were also tested for proteases activity and metabolome composition. Findings: Leishmania tarentolae does not express LPG on its surface. It expresses gp63 at lower levels compared to pathogenic species and, is highly sensitive to complement-mediated lysis. This species also lacks intracellular/extracellular activities of proteolytic enzymes. It has metabolic differences with pathogenic species, exhibiting a lower abundance of metabolites including ABC transporters, biosynthesis of unsaturated fatty acids and steroids, TCA cycle, glycine/serine/threonine metabolism, glyoxylate/dicarboxylate metabolism and pentose-phosphate pathways. Main Conclusions: The non-pathogenic phenotype of L. tarentolae is associated with alterations in several biochemical and molecular features. This reinforces the need of comparative studies between pathogenic and non-pathogenic species to elucidate the molecular mechanisms of virulence during host-parasite interactions.
Databáze:	MEDLINE
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