Technical note: Micro-computed tomography calibration using dental tissue for bone mineral research.

Autor: Towle I; Centro Nacional de Investigación sobre la Evolución Humana (CENIEH), Burgos, Spain.; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand., Loch C; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand., Oxenham M; School of Archaeology and Anthropology, Australian National University, Canberra, Australia.; Department of Archaeology, School of Geosciences, University of Aberdeen, Aberdeen, UK., Krueger KL; Department of Anthropology, Loyola University Chicago, Chicago, IL, USA., Samir Salem A; Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin, New Zealand., de Pinillos MM; Centro Nacional de Investigación sobre la Evolución Humana (CENIEH), Burgos, Spain.; Laboratorio de Evolución Humana (LEH), Universidad de Burgos, Burgos, Spain., Modesto-Mata M; Centro Nacional de Investigación sobre la Evolución Humana (CENIEH), Burgos, Spain.; Universidad Internacional de La Rioja (UNIR), Logroño La Rioja, Spain., Hlusko LJ; Centro Nacional de Investigación sobre la Evolución Humana (CENIEH), Burgos, Spain.
Jazyk: angličtina
Zdroj: American journal of biological anthropology [Am J Biol Anthropol] 2024 Jul; Vol. 184 (3), pp. e24952. Date of Electronic Publication: 2024 May 22.
DOI: 10.1002/ajpa.24952
Abstrakt: Computed tomography (CT) and microcomputed tomography (μCT) require calibration against density phantoms scanned with specimens or during routine internal calibration for assessment of mineral concentration (MC) and density. In clinical studies involving bone, alternative calibration methods using bodily tissues and fluids ("phantomless" calibration) have been suggested. However, such tissues are seldom available in archeological and osteological research. This study investigates the potential of dental tissue as internal reference for calibration of μCT scans, facilitating the analysis of bone MC. We analyzed 70 molars from 24 extant primate species, including eight human teeth, each scanned with density phantoms for calibration. Our findings indicate that sampling specific regions of molars (lateral aspects of the mesial cusps) yields low variation in enamel and dentine MC values, averaging 1.27 g/cm 3 (±0.03) for dentine and 2.25 g/cm 3 (±0.03) for enamel. No significant differences were observed across molar types or among scanning procedures, including scanner model, resolution, and filters. An ad hoc test on 12 mandibles revealed low variance in MC between the conventional phantom and dental tissue calibration methods; all 36 measurements (low, medium, and high MC for each mandible) were within 0.05 g/cm 3 of each other -81% were within 0.03 g/cm 3 and 94% within 0.04 g/cm 3 . Based on these results, we propose a new "phantomless" calibration technique using these mean enamel and dentine MC values. The presented phantomless calibration method could aid in the assessment of bone pathology and enhance the scope of studies investigating bone structure and physical property variations in archeological, osteological, and laboratory-based research.
(© 2024 The Authors. American Journal of Biological Anthropology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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