Use of topical methylene blue to image nuclear morphometry with a low-cost scanning darkfield microendoscope.
| Autor: | Hou H; Rice University, Department of Bioengineering, Houston, Texas, United States., Carns J; Rice University, Department of Bioengineering, Houston, Texas, United States., Schwarz RA; Rice University, Department of Bioengineering, Houston, Texas, United States., Gillenwater AM; The University of Texas M.D. Anderson Cancer Center, Department of Head and Neck Surgery, Houston, Texas, United States., Anandasabapathy S; Baylor College of Medicine, Department of Medicine, Houston, Texas, United States., Richards-Kortum RR; Rice University, Department of Bioengineering, Houston, Texas, United States. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of biomedical optics [J Biomed Opt] 2024 May; Vol. 29 (5), pp. 050501. Date of Electronic Publication: 2024 May 21. |
| DOI: | 10.1117/1.JBO.29.5.050501 |
| Abstrakt: | Significance: Fiber-optic microendoscopy is a promising approach to noninvasively visualize epithelial nuclear morphometry for early cancer and precancer detection. However, the broader clinical application of this approach is limited by a lack of topical contrast agents available for in vivo use. Aim: The aim of this study was to evaluate the ability to image nuclear morphometry in vivo with a novel fiber-optic microendoscope used together with topical application of methylene blue (MB), a dye with FDA approval for use in chromoendoscopy in the gastrointestinal tract. Approach: The low-cost, high-resolution microendoscope implements scanning darkfield imaging without complex optomechanical components by leveraging programmable illumination and the rolling shutter of the image sensor. We validate the integration of our system and MB staining for visualizing epithelial cell nuclei by performing ex vivo imaging on fresh animal specimens and in vivo imaging on healthy volunteers. Results: The results indicate that scanning darkfield imaging significantly reduces specular reflection and resolves epithelial nuclei with enhanced image contrast and spatial resolution compared to non-scanning widefield imaging. The image quality of darkfield images with MB staining is comparable to that of fluorescence images with proflavine staining. Conclusions: Our approach enables real-time microscopic evaluation of nuclear patterns and has the potential to be a powerful noninvasive tool for early cancer detection. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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