Comparative toxicological assessment of cigarettes and new category products via an in vitro multiplex proteomics platform.

Autor:	Tsolakos N; Protavio Ltd, Agia Paraskevi, Attiki 15341, Greece., Haswell LE; B.A.T. (Investments) Limited, Regents Park Road, Millbrook, Southampton SO15 8TL, UK., Miazzi F; B.A.T. (Investments) Limited, Regents Park Road, Millbrook, Southampton SO15 8TL, UK., Bishop E; B.A.T. (Investments) Limited, Regents Park Road, Millbrook, Southampton SO15 8TL, UK., Antoranz A; Protavio Ltd, Agia Paraskevi, Attiki 15341, Greece., Pliaka V; Protavio Ltd, Agia Paraskevi, Attiki 15341, Greece., Minia A; Protavio Ltd, Agia Paraskevi, Attiki 15341, Greece., Alexopoulos LG; Protavio Ltd, Agia Paraskevi, Attiki 15341, Greece.; Biomedical Systems Laboratory, School of Mechanical Engineering, National Technical University of Athens, Zografou 15373, Greece., Gaca M; B.A.T. (Investments) Limited, Regents Park Road, Millbrook, Southampton SO15 8TL, UK., Breheny D; B.A.T. (Investments) Limited, Regents Park Road, Millbrook, Southampton SO15 8TL, UK.
Jazyk:	angličtina
Zdroj:	Toxicology reports [Toxicol Rep] 2024 May 06; Vol. 12, pp. 492-501. Date of Electronic Publication: 2024 May 06 (Print Publication: 2024).
DOI:	10.1016/j.toxrep.2024.04.006
Abstrakt:	Cigarette smoking is a risk factor for several diseases such as cancer, cardiovascular disease (CVD), and chronic obstructive pulmonary diseases (COPD), however, the underlying mechanisms are not fully understood. Alternative nicotine products with reduced risk potential (RRPs) including tobacco heating products (THPs), and e-cigarettes have recently emerged as viable alternatives to cigarettes that may contribute to the overall strategy of tobacco harm reduction due to the significantly lower levels of toxicants in these products' emissions as compared to cigarette smoke. Assessing the effects of RRPs on biological responses is important to demonstrate the potential value of RRPs towards tobacco harm reduction. Here, we evaluated the inflammatory and signaling responses of human lung epithelial cells to aqueous aerosol extracts (AqE) generated from the 1R6F reference cigarette, the glo™ THP, and the Vype ePen 3.0 e-cigarette using multiplex analysis of 37 inflammatory and phosphoprotein markers. Cellular exposure to the different RRPs and 1R6F AqEs resulted in distinct response profiles with 1R6F being the most biologically active followed by glo™ and ePen 3.0. 1R6F activated stress-related and pro-survival markers c-JUN, CREB1, p38 MAPK and MEK1 and led to the release of IL-1α. glo™ activated MEK1 and decreased IL-1β levels, whilst ePen 3.0 affected IL-1β levels but had no effect on the signaling activity compared to untreated cells. Our results demonstrated the reduced biological effect of RRPs and suggest that targeted analysis of inflammatory and cell signaling mediators is a valuable tool for the routine assessment of RRPs. Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Authors.)
Databáze:	MEDLINE
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