Whole-genome sequences reveal zygotic composition in chimeric twins.

Autor:	Yoon CJ; Research Center for Natural Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA; McDonnell Genome Institute, St. Louis, MO, USA., Nam CH; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea., Kim T; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea., Lee JS; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Inocras, Inc., San Diego, CA, USA., Kim R; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Inocras, Inc., San Diego, CA, USA., Yi K; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Inocras, Inc., San Diego, CA, USA., Koh JY; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Inocras, Inc., San Diego, CA, USA., Kim J; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea., Won H; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea., Oh JW; Department of Anatomy, Yonsei University College of Medicine, Seoul, Republic of Korea., Griffith OL; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA; McDonnell Genome Institute, St. Louis, MO, USA., Griffith M; Department of Medicine, Washington University School of Medicine, St. Louis, MO, USA; McDonnell Genome Institute, St. Louis, MO, USA., Sung J; Department of Epidemiology, School of Public Health, Seoul National University, Seoul, Korea., Kim TY; Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea., Cho D; Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. Electronic address: duck.cho@skku.edu., Choi JS; Department of Laboratory Medicine, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon, Korea. Electronic address: sunnychoi@ish.ac.kr., Ju YS; Research Center for Natural Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea; Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, Korea. Electronic address: ysju@kaist.ac.kr.
Jazyk:	angličtina
Zdroj:	HGG advances [HGG Adv] 2024 Jul 18; Vol. 5 (3), pp. 100301. Date of Electronic Publication: 2024 May 21.
DOI:	10.1016/j.xhgg.2024.100301
Abstrakt:	While most dizygotic twins have a dichorionic placenta, rare cases of dizygotic twins with a monochorionic placenta have been reported. The monochorionic placenta in dizygotic twins allows in utero exchange of embryonic cells, resulting in chimerism in the twins. In practice, this chimerism is incidentally identified in mixed ABO blood types or in the presence of cells with a discordant sex chromosome. Here, we applied whole-genome sequencing to one triplet and one twin family to precisely understand their zygotic compositions, using millions of genomic variants as barcodes of zygotic origins. Peripheral blood showed asymmetrical contributions from two sister zygotes, where one of the zygotes was the major clone in both twins. Single-cell RNA sequencing of peripheral blood tissues further showed differential contributions from the two sister zygotes across blood cell types. In contrast, buccal tissues were pure in genetic composition, suggesting that in utero cellular exchanges were confined to the blood tissues. Our study illustrates the cellular history of twinning during human development, which is critical for managing the health of chimeric individuals in the era of genomic medicine. Competing Interests: Declaration of interests Y.S.J. is the co-founder and chief genomics officer of Inocras, Inc. J.S.L. is the co-founder and chief innovation officer of Inocras, Inc. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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