Pathological and phylogenetic characteristics of fowl AOAV-1 and H5 isolated from naturally infected Meleagris Gallopavo.

Autor: Shalaby S; Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura City, 35516, Egypt. shadyshalaby25@mans.edu.eg., Awadin W; Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura City, 35516, Egypt. awadin@mans.edu.eg., Manzoor R; Veterinary Science Program, Faculty of Health Sciences, Higher Colleges of Technology, P.O. Box 7946, Sharjah City, UAE. rmanzoor@hct.ac.ae., Karam R; Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura City, 35516, Egypt., Mohamadin M; Veterinary Science Program, Faculty of Health Sciences, Higher Colleges of Technology, P.O. Box 7946, Sharjah City, UAE., Salem S; Department of Pathology, Zagazig Branch, Agriculture Research Centre (ARC), Animal Health Research Institute (AHRI), P.O. Box 44516, Zagazig City, Egypt., El-Shaieb A; Department of Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansoura City, 35516, Egypt.; Faculty of Veterinary Medicine, Egyptian Chinese University, Ain Shams City, 4541312, Egypt.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 May 21; Vol. 20 (1), pp. 216. Date of Electronic Publication: 2024 May 21.
DOI: 10.1186/s12917-024-04029-4
Abstrakt: Background: In this study, we investigated the prevalence of respiratory viruses in four Hybrid Converter Turkey (Meleagris gallopavo) farms in Egypt. The infected birds displayed severe respiratory signs, accompanied by high mortality rates, suggesting viral infections. Five representative samples from each farm were pooled and tested for H5 & H9 subtypes of avian influenza viruses (AIVs), Avian Orthoavulavirus-1 (AOAV-1), and turkey rhinotracheitis (TRT) using real-time RT-PCR and conventional RT-PCR. Representative tissue samples from positive cases were subjected to histopathology and immunohistochemistry (IHC).
Results: The PCR techniques confirmed the presence of AOAV-1 and H5 AIV genes, while none of the tested samples were positive for H9 or TRT. Microscopic examination of tissue samples revealed congestion and hemorrhage in the lungs, liver, and intestines with leukocytic infiltration. IHC revealed viral antigens in the lungs, liver, and intestines. Phylogenetic analysis revealed that H5 HA belonged to 2.3.4.4b H5 sublineage and AOAV-1 belonged to VII 1.1 genotype.
Conclusions: The study highlights the need for proper monitoring of hybrid converter breeds for viral diseases, and the importance of vaccination programs to prevent unnecessary losses. To our knowledge, this is the first study that reports the isolation of AOAV-1 and H5Nx viruses from Hybrid Converter Turkeys in Egypt.
(© 2024. The Author(s).)
Databáze: MEDLINE
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