Exploring the mechanism of transformation in Acacia nilotica (Linn.) triggered by colchicine seed treatment.

Autor: Haider MW; Department of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan. wasim.haider@iub.edu.pk., Nafees M; Department of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan., Iqbal R; Department of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur Pakistan, Bahawalpur, 63100, Pakistan., Asad HU; Centre for Agriculture and Bioscience International, Rawalpindi, 46300, Pakistan., Azeem F; Agri Development, Fauji Fresh N Freeze Ltd, Gulberg II, Lahore, 48000, Pakistan., Raza MS; Department of Horticultural Sciences, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan., Gaafar AZ; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia., Elshikh MS; Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia., Arslan M; Department of Agroecology and Organic Farming, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, 53115, Germany. m.arslan@uni-bonn.de., Rahman MHU; Institute of Crop Science and Resource Conservation (INRES), Crop Science, University of Bonn, 53115, Bonn, Germany., Elshamly AMS; Water Studies and Research Complex, National Water Research Center, Cairo, Egypt.
Jazyk: angličtina
Zdroj: BMC plant biology [BMC Plant Biol] 2024 May 21; Vol. 24 (1), pp. 428. Date of Electronic Publication: 2024 May 21.
DOI: 10.1186/s12870-024-05139-9
Abstrakt: Background: Acacia nilotica Linn. is a widely distributed tree known for its applications in post-harvest and medicinal horticulture. However, its seed-based growth is relatively slow. Seed is a vital component for the propagation of A. nilotica due to its cost-effectiveness, genetic diversity, and ease of handling. Colchicine, commonly used for polyploidy induction in plants, may act as a pollutant at elevated levels. Its optimal concentration for Acacia nilotica's improved growth and development has not yet been determined, and the precise mechanism underlying this phenomenon has not been established. Therefore, this study investigated the impact of optimized colchicine (0.07%) seed treatment on A. nilotica's morphological, anatomical, physiological, fluorescent, and biochemical attributes under controlled conditions, comparing it with a control.
Results: Colchicine seed treatment significantly improved various plant attributes compared to control. This included increased shoot length (84.6%), root length (53.5%), shoot fresh weight (59.1%), root fresh weight (42.8%), shoot dry weight (51.5%), root dry weight (40%), fresh biomass (23.6%), stomatal size (35.9%), stomatal density (41.7%), stomatal index (51.2%), leaf thickness (11 times), leaf angle (2.4 times), photosynthetic rate (40%), water use efficiency (2.2 times), substomatal CO 2 (36.6%), quantum yield of photosystem II (13.1%), proton flux (3.1 times), proton conductivity (2.3 times), linear electron flow (46.7%), enzymatic activities of catalase (25%), superoxide dismutase (33%), peroxidase (13.5%), and ascorbate peroxidase (28%), 2,2-diphenyl-1-picrylhydrazyl-radical scavenging activities(23%), total antioxidant capacity (59%), total phenolic (23%), and flavonoid content (37%) with less number of days to 80% germination (57.1%), transpiration rate (53.9%), stomatal conductance (67.1%), non-photochemical quenching (82.8%), non-regulatory energy dissipation (24.3%), and H 2 O 2 (25%) and O -2 levels (30%).
Conclusion: These findings elucidate the intricate mechanism behind the morphological, anatomical, physiological, fluorescent, and biochemical transformative effects of colchicine seed treatment on Acacia nilotica Linn. and offer valuable insights for quick production of A. nilotica's plants with modification and enhancement from seeds through an eco-friendly approach.
(© 2024. The Author(s).)
Databáze: MEDLINE
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