Biochemical parameters, oxidative stress biomarkers, and anatomopathological changes in Wistar rats treated with 3'-demethoxy-6-O-demethylisoguaiacin and norisoguaiacin.

Autor: Flores Jiménez NG; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Palo Alto, Mexico, Mexico., Zamorano MM; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., Reséndiz-González G; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., Mercado-Márquez C; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., Morales-Álvarez JF; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Palo Alto, Mexico, Mexico., Cuéllar-Ordaz JA; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., de-la-Cruz Cruz HA; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., Adams-Vázquez O; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., López-Arellano ME; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Jiutepec, Morelos, Mexico., Díaz-Torres R; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., Noguera PR; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico., Higuera-Piedrahita RI; Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Mexico, Estado de México, Mexico. rhiguera05@comunidad.unam.mx.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 21; Vol. 14 (1), pp. 11568. Date of Electronic Publication: 2024 May 21.
DOI: 10.1038/s41598-024-61903-9
Abstrakt: Artemisia cina (Ac) is a plant with anthelmintic compounds such as 3'-demethoxy-6-O-demethylisoguaiacin (D) and norisoguaiacin (N). Three major objectives were proposed: (1) To evaluate biochemical parameters in blood (2) to determine the tissue oxidative stress by biomarkers as TBARS and glutathione peroxidase activity, and (3) to evaluate anatomopathological changes in organs such as the brain, liver, kidney, and lung after oral administration of n-hexane extract of Ac and D and N. D and N were administrated following the OECD guides for acute oral toxicity evaluation (Guide 420). Fifty Wistar rats were distributed into ten groups as follows: Group 1 (G1): 4 mg/Kg; G2: 40 mg/Kg; G3: 240 mg/Kg; G4: 1600 mg/Kg of n-hexane extract of Ac. G5: 2 mg/Kg; G6: 20 mg/Kg; G7: 120 mg/Kg; G8: 800 mg/Kg of D and N, G9: water and G10: polyvinylpyrrolidone at 2000 mg/Kg. At 14 days, the rats were euthanized, and the blood, liver, brain, kidney, and lung were taken for biochemical analysis, anatomopathological changes, and TBARS and GSH evaluation. Glucose, cholesterol, and phosphorus were altered. Histopathological analysis showed multifocal neuronal degeneration in the brain (G2). The kidney and lungs had changes in G7. The GSH and TBARS increased in G6 and G7. The TBARS activity was higher in G1 and G2. In conclusion, extract and D and N of Ac did not have damage at therapeutic doses. D, N, and n-hexane extract of A. cina do not cause histopathological damage at pharmaceutical doses. Still, the brain, kidney, and liver are related to biochemical parameters at higher doses. However, compounds are proposed as antioxidant agents.
(© 2024. The Author(s).)
Databáze: MEDLINE
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