Evaluating the antimicrobial and anti-biofilm activity of three synthetic antimicrobial Citropin analogs and their ability to fight against Staphylococcus aureus and Staphylococcus pseudintermedius.

Autor:	Salvado MG; Graduate Program in Veterinary Medicine, Fluminense Federal University, Niteroi, 24230-321, Brazil.; Gram Positive Cocci Laboratory, Fluminense Federal University, Niteroi, 24020-141, Brazil., André LSP; Molecular Epidemiology and Biotechnology Laboratory/Rodolpho Albino University Laboratory - Fluminense Federal University, Niteroi, 24241-000, Brazil.; Graduate Program in Pathology, Fluminense Federal University, Niteroi 24020-141, Brazil., Pereira RFA; Molecular Epidemiology and Biotechnology Laboratory/Rodolpho Albino University Laboratory - Fluminense Federal University, Niteroi, 24241-000, Brazil.; Graduate Program in Microbiology and Parasitology - Fluminense Federal University, Niteroi, 24020-141, Brazil., Pinheiro FR; Molecular Epidemiology and Biotechnology Laboratory/Rodolpho Albino University Laboratory - Fluminense Federal University, Niteroi, 24241-000, Brazil.; Graduate Program in Pathology, Fluminense Federal University, Niteroi 24020-141, Brazil., Barbosa BDC; Molecular Epidemiology and Biotechnology Laboratory/Rodolpho Albino University Laboratory - Fluminense Federal University, Niteroi, 24241-000, Brazil., Scaffo JC; Molecular Epidemiology and Biotechnology Laboratory/Rodolpho Albino University Laboratory - Fluminense Federal University, Niteroi, 24241-000, Brazil., Pereira AJ; Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, United States., Arakaki DG; Group of Spectroscopy and Bioinformatics Applied Biodiversity and Health, Mato Grosso do Sul Federal University, Pioneiros, 79070-900, Brazil., Xing H; Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, United States., de Oliveira KMP; Microbiological assays laboratory, Great Dourados Federal University, Dourados, 79.804-970, Brazil., de Andrade Dos Santos JV; Microbiological assays laboratory, Great Dourados Federal University, Dourados, 79.804-970, Brazil., Sachs D; Microbiological Testing Laboratory Associated with Materials and Drugs of the Center for Studies, Research, and Innovation in Biofunctional Materials and Biotechnology, Federal University of Itajubá, Itajuba, 37500-903, Brazil., Aguiar-Alves F; Department of Pharmaceutical Sciences, Lloyd L. Gregory School of Pharmacy, Palm Beach Atlantic University, West Palm Beach, FL 33403, USA., Conda-Sheridan M; Department of Pharmaceutical Sciences, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198, United States., Penna B; Graduate Program in Veterinary Medicine, Fluminense Federal University, Niteroi, 24230-321, Brazil.; Gram Positive Cocci Laboratory, Fluminense Federal University, Niteroi, 24020-141, Brazil.; Graduate Program in Microbiology and Parasitology - Fluminense Federal University, Niteroi, 24020-141, Brazil.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2024 Jun 03; Vol. 135 (6).
DOI:	10.1093/jambio/lxae127
Abstrakt:	Aims: We developed three new analogs of the antimicrobial peptide (AMP) Citropin 1.1: DAN-1-13, AJP-1-1, and HHX-2-28, and tested their potential antimicrobial and antibiofilm activities against Staphylococcus aureus and S. pseudintermedius. Potential cytotoxic or hemolytic effects were determined using cultured human keratinocytes and erythrocytes to determine their safety. Methods and Results: To assess the antimicrobial activity of each compound, minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined against methicillin-resistant and methicillin-susceptible strains of S. aureus and S. pseudintermedius. Activity against newly formed and mature biofilms was determined in two clinical isolates using spectrophotometry and scanning electron microscopy (SEM). All three compounds exhibited antimicrobial and bactericidal activity against all studied S. aureus and S. pseudintermedius strains, with MICs ranging from 4-32 μg ml-1 and MBCs ranging from 8-128 μg ml-1. Subinhibitory concentrations of all compounds also showed ant-biofilm activity in the two tested isolates. All compounds exhibited limited cytotoxic and hemolytic activity. Conclusions: Novel analogs of Citropin 1.1 exhibit antimicrobial and bactericidal activities against S. aureus and S. pseudintermedius isolates and inhibit the biofilm formation of these bacteria. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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