Engineering Bacillus subtilis for highly efficient production of functional disaccharide lactulose from lactose.

Autor: Zhang W; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China., Xiong S; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming 650500, China., Ni D; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China., Huang Z; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China., Ding J; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Yunnan Normal University, Kunming 650500, China., Mu W; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: wmmu@jiangnan.edu.cn.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Jun; Vol. 271 (Pt 1), pp. 132478. Date of Electronic Publication: 2024 May 19.
DOI: 10.1016/j.ijbiomac.2024.132478
Abstrakt: Bioconversion of lactose to functional lactose derivatives attracts increasing attention. Lactulose is an important high-value lactose derivative, which has been widely used in pharmaceutical, nutraceutical, and food industries. Lactulose can be enzymatically produced from lactose by cellobiose 2-epimerase (CEase). Several studies have already focused on the food-grade expression of CEase, but they are all aimed at the biosynthesis of epilactose. Herein, we reported for the first time the biosynthesis of lactulose using the recombinant food-grade Bacillus subtilis. Lactulose biosynthesis was optimized by varying lactulose-producing CEases and expression vectors. Caldicellulosiruptor saccharolyticus CEase and pP43NMK were determined to be the optimal CEase and expression vector. Fine-tuning of CEase expression was investigated by screening a beneficial N-terminal coding sequence. After fed-batch cultivation, the highest fermentation isomerization activity reached 11.6 U/mL. Lactulose was successfully produced by the broth of the engineered B. subtilis with a yield of 52.1 %.
Competing Interests: Declaration of competing interest The authors declare that they have no known financial interests or personal relationships that could have influenced the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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