Extraction of Pithecellobium clypearia Benth polysaccharides by dual-frequency ultrasound-assisted extraction: Structural characterization, antioxidant, hypoglycemic and anti-hyperlipidemic activities.

Autor: Chen Z; School of Pharmacy, Guangdong Pharmaceutical University, China., Wang C; School of Pharmacy, Guangdong Pharmaceutical University, China., Su J; School of Pharmacy, Guangdong Pharmaceutical University, China., Liang G; School of Pharmacy, Guangdong Pharmaceutical University, China., Tan S; Guangdong Dongshenglin Pharmaceutical Co., Ltd, China., Bi Y; School of Pharmacy, Guangdong Pharmaceutical University, China; Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, China; Guangdong Dongshenglin Pharmaceutical Co., Ltd, China; Yunfu Traditional Chinese Medicine Hospital, China. Electronic address: biyongguang2002@163.com., Kong F; School of Pharmacy, Guangdong Pharmaceutical University, China., Wang Z; Yunfu Traditional Chinese Medicine Hospital, China.
Jazyk: angličtina
Zdroj: Ultrasonics sonochemistry [Ultrason Sonochem] 2024 Jul; Vol. 107, pp. 106918. Date of Electronic Publication: 2024 May 18.
DOI: 10.1016/j.ultsonch.2024.106918
Abstrakt: In this research, the extraction process of polysaccharides from Pithecellobium clypearia Benth (PCBPs) was optimized using dual-frequency ultrasound-assisted extraction (DUAE). The biological activities of PCBPs were investigated by in vitro antioxidant, hypoglycemic, and anti-hyperlipidemic assay. High-performance anion-exchange chromatography, high-performance gel permeation chromatography, SEM, UV-Vis spectroscopy, and FT-IR spectra were used to analyze the monosaccharide composition, molecular weight, microscopic morphology, and characteristic structure of PCBPs. The results showed that the maximum extraction rate of PCBPs was 9.90 ± 0.16% when the ultrasonic time was 8 min, the liquid-to-material ratio was 32 mL/g, and the ultrasonic power was 510 W. The PCBPs also possessed excellent in vitro antioxidant, hypoglycemic, and anti-hyperlipidemic activities. In addition, the average molecular weight of PCBPs was 15.07 kDa. PCBPs consisted of rhamnose, arabinose, galactose, glucose, xylose, mannose, and glucuronic acid, with the molar ratios of 11.07%, 18.54%, 48.17%, 10.44%, 4.62%, 4.96%, and 2.20%, respectively. Moreover, the results of SEM showed that PCBPs mainly showed a fine spherical mesh structure. The above studies provided a valuable theoretical basis for the subsequent in-depth study of PCBPs.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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