Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris.

Autor: Qiao H; The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China., Chen J; The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China., Yang S; The College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, China.
Jazyk: angličtina
Zdroj: Journal of basic microbiology [J Basic Microbiol] 2024 Jul; Vol. 64 (7), pp. e2400103. Date of Electronic Publication: 2024 May 21.
DOI: 10.1002/jobm.202400103
Abstrakt: This study aimed to enhance extracellular polysaccharide (EPS) production in Cordyceps militaris by constructing a quorum sensing (QS) system to regulate the expression of biosynthetic enzyme genes, including phosphoglucomutase, hexokinase, phosphomannomutase, polysaccharide synthase, and UDP-glucose 4-epimerase genes. The study found higher EPS concentrations in seven recombinant strains compared to the wild-type C. militaris, indicating that the overexpression of key enzyme genes increased EPS production. Among them, the CM-pgm-2 strain exhibited the highest EPS production, reaching a concentration of 3.82 ± 0.26 g/L, which was 1.52 times higher than the amount produced by the wild C. militaris strain. Additionally, the regulatory effects of aromatic amino acids on the QS system of the CM-pgm-2 strain were investigated. Under the influence of 45 mg/L tryptophan, the EPS production in CM-pgm-2 reached 4.75 ± 0.20 g/L, representing a 1.90-fold increase compared to wild C. militaris strains. This study provided an effective method for the large-scale production of EPSs in C. militaris, and opened up new avenues for research into fungal QS mechanisms.
(© 2024 Wiley‐VCH GmbH.)
Databáze: MEDLINE
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