Disrupted one-carbon metabolism in heifers negatively affects their health and physiology.

Autor:	Crouse MS; USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA., Trotta RJ; Department of Animal and Food Sciences, University of Kentucky, Lexington, KY 40546, USA., Freetly HC; USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA., Lindholm-Perry AK; USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA., Neville BW; USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA., Oliver WT; USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA., Hammer CJ; Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA., Syring JG; Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA., King LE; Department of Agriculture and Natural Resources, University of Minnesota Crookston, Crookston, MN 56716, USA., Neville TL; Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA., Reynolds LP; Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA., Dahlen CR; Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA., Caton JS; Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58108, USA., Ward AK; Department of Veterinary Biomedical Science, University of Saskatchewan, Saskatoon, SK S7N5A2, Canada., Cushman RA; USDA, ARS, U.S. Meat Animal Research Center, Clay Center, NE 68933, USA.
Jazyk:	angličtina
Zdroj:	Journal of animal science [J Anim Sci] 2024 Jan 03; Vol. 102.
DOI:	10.1093/jas/skae144
Abstrakt:	The objective of this study was to determine the dose-dependent response of one-carbon metabolite (OCM: methionine, choline, folate, and vitamin B12) supplementation on heifer dry matter intake on fixed gain, organ mass, hematology, cytokine concentration, pancreatic and jejunal enzyme activity, and muscle hydrogen peroxide production. Angus heifers (n = 30; body weight [BW] = 392.6 ± 12.6 kg) were individually fed and assigned to one of five treatments: 0XNEG: total mixed ration (TMR) and saline injections at days 0 and 7 of the estrous cycle, 0XPOS: TMR, rumen-protected methionine (MET) fed at 0.08% of the diet dry matter, rumen-protected choline (CHOL) fed at 60 g/d, and saline injections at days 0 and 7, 0.5X: TMR, MET, CHOL, 5-mg B12, and 80-mg folate injections at days 0 and 7, 1X: TMR, MET CHOL, 10-mg vitamin B12, and 160-mg folate at days 0 and 7, and 2X: TMR, MET, CHOL, 20-mg vitamin B12, and 320-mg folate at days 0 and 7. All heifers were estrus synchronized but not bred, and blood samples were collected on days 0, 7, and at slaughter (day 14) during which tissues were collected. By design, heifer ADG did not differ (P = 0.96). Spleen weight and uterine weight were affected cubically (P = 0.03) decreasing from 0XPOS to 0.5X. Ovarian weight decreased linearly (P < 0.01) with increasing folate and B12 injection. Hemoglobin and hematocrit percentage were decreased (P < 0.01) in the 0.5X treatment compared with all other treatments. Plasma glucose, histotroph protein, and pancreatic α-amylase were decreased (P ≤ 0.04) in the 0.5X treatment. Heifers on the 2X treatment had greater pancreatic α-amylase compared with 0XNEG and 0.5X treatment. Interleukin-6 in plasma tended (P = 0.08) to be greater in the 0XPOS heifers compared with all other treatments. Lastly, 0XPOS-treated heifers had reduced (P ≤ 0.07) hydrogen peroxide production in muscle compared with 0XNEG heifers. These data imply that while certain doses of OCM do not improve whole animal physiology, OCM supplementation doses that disrupt one-carbon metabolism, such as that of the 0.5X treatment, can induce a negative systemic response that results in negative effects in both the dam and the conceptus during early gestation. Therefore, it is necessary to simultaneously establish an optimal OCM dose that increases circulating concentrations for use by the dam and the conceptus, while avoiding potential negative side effects of a disruptive OCM, to evaluate the long-term impacts of OCM supplementation of offspring programming. (© The Author(s) 2024. Published by Oxford University Press on behalf of the American Society of Animal Science.)
Databáze:	MEDLINE
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