In vitro effects of wound-dressings on key wound healing properties of dermal fibroblasts.

Autor: Peltier S; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France., Adib Y; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France., Nicosia L; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France., Ly Ka So S; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France., Da Silva C; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France., Serror K; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France.; Service de Chirurgie plastique, reconstructive et esthétique, APHP, Hôpital Saint-Louis, Paris, France., Duciel L; Laboratoires Brothier, Nanterre, France., Proust R; Laboratoires Brothier, Nanterre, France., Mimoun M; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France.; Service de Chirurgie plastique, reconstructive et esthétique, APHP, Hôpital Saint-Louis, Paris, France., Bagot M; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France.; Service de Dermatologie, APHP, Hôpital Saint Louis, Paris, France., Bensussan A; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France., des Courtils C; Laboratoires Brothier, Nanterre, France., Michel L; Inserm UMRS_976, Hôpital Saint-Louis, Paris, France.; Université Paris Cité, Paris, France.; Service de Dermatologie, APHP, Hôpital Saint Louis, Paris, France.
Jazyk: angličtina
Zdroj: Experimental dermatology [Exp Dermatol] 2024 May; Vol. 33 (5), pp. e15098.
DOI: 10.1111/exd.15098
Abstrakt: Healing of complex wounds requires dressings that must, at least, not hinder and should ideally promote the activity of key healing cells, in particular fibroblasts. This in vitro study assessed the effects of three wound-dressings (a pure Ca 2+ alginate: Algostéril®, a Ca 2+ alginate + carboxymethylcellulose: Biatain alginate® and a polyacrylate impregnated with lipido-colloid matrix: UrgoClean®) on dermal fibroblast activity. The results showed the pure calcium alginate to be non-cytotoxic, whereas the other wound-dressings showed moderate to strong cytotoxicity. The two alginates stimulated fibroblast migration and proliferation, whereas the polyacrylate altered migration and had no effect on proliferation. The pure Ca 2+ alginate significantly increased the TGF-β-induced fibroblast activation, which is essential to healing. This activation was confirmed by a significant increase in Vascular endothelial growth factor (VEGF) secretion and a higher collagen production. The other dressings reduced these fibroblast activities. The pure Ca 2+ alginate was also able to counteract the inhibitory effect of NK cell supernatants on fibroblast migration. These in vitro results demonstrate that tested wound-dressings are not equivalent for fibroblast activation. Only Algostéril was found to promote all the fibroblast activities tested, which could contribute to its healing efficacy demonstrated in the clinic.
(© 2024 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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