Study of the anti-cancer activity of a mesoporous silica nanoparticle surface coated with polydopamine loaded with umbelliprenin.

Autor: Edalatian Tavakoli S; Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran., Motavalizadehkakhky A; Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran. amotavalizadeh@yahoo.com., Homayouni Tabrizi M; Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran., Mehrzad J; Department of Biochemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran., Zhiani R; Department of Chemistry, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 20; Vol. 14 (1), pp. 11450. Date of Electronic Publication: 2024 May 20.
DOI: 10.1038/s41598-024-62409-0
Abstrakt: A mesoporous silica nanoparticle (MSN) coated with polydopamine (PDA) and loaded with umbelliprenin (UMB) was prepared and evaluated for its anti-cancer properties in this study. Then UMB-MSN-PDA was characterized by dynamic light scattering (DLS), Field emission scanning electron microscopy (FESEM), Transmission electron microscopy (TEM) and FTIR methods. UV-visible spectrometry was employed to study the percentage of encapsulation efficiency (EE%). UMB-MSN-PDA mediated cell cytotoxicity and their ability to induce programmed cell death were evaluated by MTT, real-time qPCR, flow cytometry, and AO/PI double staining methods. The size of UMB-MSN-PDA was 196.7 with a size distribution of 0.21 and a surface charge of -41.07 mV. The EE% was 91.92%. FESEM and TEM showed the spherical morphology of the UMB-MSN-PDA. FTIR also indicated the successful interaction of the UMB and MSN and PDA coating. The release study showed an initial 20% release during the first 24 h of the study and less than 40% during 168 h. The lower cytotoxicity of the UMB-MSN-PDA against HFF normal cells compared to MCF-7 carcinoma cells suggested the safety of formulation on normal cells and tissues. The induction of apoptosis in MCF-7 cells was indicated by the upregulation of P53, caspase 8, and caspase 9 genes, enhanced Sub-G1 phase cells, and the AO/PI fluorescent staining. As a result of these studies, it may be feasible to conduct preclinical studies shortly to evaluate the formulation for its potential use in cancer treatment.
(© 2024. The Author(s).)
Databáze: MEDLINE
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