AtHVA22a, a plant-specific homologue of Reep/DP1/Yop1 family proteins is involved in turnip mosaic virus propagation.

Autor: Xue M; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Sofer L; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Simon V; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Arvy N; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Diop M; UR 1052, INRAe, GAFL Domaine St Maurice, Montfavet Cedex, France., Lion R; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Beucher G; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Bordat A; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France., Tilsner J; Cell and Molecular Sciences, James Hutton Institute, Dundee, UK.; Biomedical Sciences Research Complex, University of St Andrews, St Andrews, UK., Gallois JL; UR 1052, INRAe, GAFL Domaine St Maurice, Montfavet Cedex, France., German-Retana S; Univ. Bordeaux UMR 1332, Biologie du Fruit et Pathologie, INRAe, Equipe de Virologie, Villenave d'Ornon Cedex, France.
Jazyk: angličtina
Zdroj: Molecular plant pathology [Mol Plant Pathol] 2024 May; Vol. 25 (5), pp. e13466.
DOI: 10.1111/mpp.13466
Abstrakt: The movement of potyviruses, the largest genus of single-stranded, positive-sense RNA viruses responsible for serious diseases in crops, is very complex. As potyviruses developed strategies to hijack the host secretory pathway and plasmodesmata (PD) for their transport, the goal of this study was to identify membrane and/or PD-proteins that interact with the 6K2 protein, a potyviral protein involved in replication and cell-to-cell movement of turnip mosaic virus (TuMV). Using split-ubiquitin membrane yeast two-hybrid assays, we screened an Arabidopsis cDNA library for interactors of TuMV 6K2. We isolated AtHVA22a (Hordeum vulgare abscisic acid responsive gene 22), which belongs to a multigenic family of transmembrane proteins, homologous to Receptor expression-enhancing protein (Reep)/Deleted in polyposis (DP1)/Yop1 family proteins in animal and yeast. HVA22/DP1/Yop1 family genes are widely distributed in eukaryotes, but the role of HVA22 proteins in plants is still not well known, although proteomics analysis of PD fractions purified from Arabidopsis suspension cells showed that AtHVA22a is highly enriched in a PD proteome. We confirmed the interaction between TuMV 6K2 and AtHVA22a in yeast, as well as in planta by using bimolecular fluorescence complementation and showed that TuMV 6K2/AtHVA22a interaction occurs at the level of the viral replication compartment during TuMV infection. Finally, we showed that the propagation of TuMV is increased when AtHVA22a is overexpressed in planta but slowed down upon mutagenesis of AtHVA22a by CRISPR-Cas9. Altogether, our results indicate that AtHVA22a plays an agonistic effect on TuMV propagation and that the C-terminal tail of the protein is important in this process.
(© 2024 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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