| Autor: |
Arul D; Department of Biotechnology, School of Biosciences, Periyar University, Salem, India.; Division of Crop Production, ICAR - Indian Institute of Horticulture Research, Hesaraghatta, Bengaluru, India., Vinoth G; Department of Chemistry, School of Physical Sciences, Periyar University, Salem, India., Muthusamy R; PG and Research Centre in Biotechnology, MGR College, Adhiyamaan Educational Research Institution, Hosur, India., Natarajan T; Department of Biotechnology, School of Biosciences, Periyar University, Salem, India., Kamaraj C; Department of Biotechnology, School of Biosciences, Periyar University, Salem, India.; Interdisciplinary Institute of Indian System of Medicine (IIISM), 5th Floor SRM Institute of Science and Technology (SRMIST), Chennai, India., Deepak P; Department of Biotechnology, School of Biosciences, Periyar University, Salem, India.; Department of Life Science, Kristu Jayanti College (Autonomous), Affiliated to Bengaluru North University, Bengaluru, India., Dhanasundaramd S; Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, India., Perumal P; Department of Biotechnology, School of Biosciences, Periyar University, Salem, India.; Department of Marine Science, School of Marine Sciences, Bharathidasan University, Tiruchirappalli, India., Ramkumar G; Department of Entomology, University of Georgia, Athens, GA, USA. |
| Abstrakt: |
The targeted organisms include mosquito vectors, bacterial pathogens and non-targeted organisms. Preliminary mosquito larvicidal activity was conducted using cell-free supernatants (CFSs) from 11 gut bacteria. Among them, the bacterium SS11 exhibited promising results and was identified as Kurthia gibsonii based on its 16S rRNA sequence (1350 bp). The diethyl ether extract (DEE) of K. gibsonii demonstrated significant larvicidal effects, with LC50 values of 5.59 µL/mL and 8.59 µL/mL for 3rd instar larvae of Aedes aegypti and 2nd instar larvae of Anopheles stephensi , respectively. Analysis of the DEE using FT-IR, and GC-MS revealed the presence of 16 functional groups, and 7 bioactive compounds, respectively. A molecular docking study identified GC-MS compounds against odorant receptors from A. aegypti and odorant-binding proteins from A. stephensi was performed to assess the interaction and binding affinity. Overall, these findings suggest that the bioactive compounds 2, 4, 6-tribromoaniline from the DEE of K. gibsonii hold potential as an environmentally compatible alternative for biocontrol purposes, and compounds 9-tricosene and didecyl phthalate can be used for mosquito traps. |
