| Autor: |
Qiu GL; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China., Peng LJ; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China., Wang P; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China., Yang ZL; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China., Zhang JQ; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China., Liu H; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China., Zhu XN; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. E-mail: zhuxn@shanghaitech.edu.cn., Rao J; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China. E-mail: 18256954080@163.com., Liu XS; Department of Anesthesiology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, China. E-mail: liuxuesheng@ahmu.edu.cn. |
| Abstrakt: |
General anesthesia is widely applied in clinical practice. However, the precise mechanism of loss of consciousness induced by general anesthetics remains unknown. Here, we measured the dynamics of five neurotransmitters, including γ-aminobutyric acid, glutamate, norepinephrine, acetylcholine, and dopamine, in the medial prefrontal cortex and primary visual cortex of C57BL/6 mice through in vivo fiber photometry and genetically encoded neurotransmitter sensors under anesthesia to reveal the mechanism of general anesthesia from a neurotransmitter perspective. Results revealed that the concentrations of γ-aminobutyric acid, glutamate, norepinephrine, and acetylcholine increased in the cortex during propofol-induced loss of consciousness. Dopamine levels did not change following the hypnotic dose of propofol but increased significantly following surgical doses of propofol anesthesia. Notably, the concentrations of the five neurotransmitters generally decreased during sevoflurane-induced loss of consciousness. Furthermore, the neurotransmitter dynamic networks were not synchronized in the non-anesthesia groups but were highly synchronized in the anesthetic groups. These findings suggest that neurotransmitter dynamic network synchronization may cause anesthetic-induced loss of consciousness. |
