A series of spontaneously blinking dyes for super-resolution microscopy.

Autor:	Holland KL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Plutkis SE; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Daugird TA; Department of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA., Sau A; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA., Grimm JB; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., English BP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Zheng Q; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Dave S; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA., Rahman F; Joint Department of Biomedical Engineering, University of North Carolina-Chapel Hill and North Carolina State University, Chapel Hill, NC, USA., Xie L; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Dong P; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Tkachuk AN; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Brown TA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Singer RH; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA., Liu Z; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA., Galbraith CG; Department of Biomedical Engineering and Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA., Musser SM; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA., Legant WR; Department of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.; Joint Department of Biomedical Engineering, University of North Carolina-Chapel Hill and North Carolina State University, Chapel Hill, NC, USA., Lavis LD; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 24. Date of Electronic Publication: 2024 Feb 24.
DOI:	10.1101/2024.02.23.581625
Abstrakt:	Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures. Competing Interests: DECLARATIONS OF INTERESTS US Patents 9,933,417 and 11,091,643 and US Patent Applications 2021/0171490 and 2021/0085805 describing azetidine-containing fluorophores and variant compositions (with inventors J.B.G., Q.Z., and L.D.L.) are assigned to HHMI. L.D.L. is a founder and shareholder of Eikon Therapeutics. The authors declare no other competing interests.
Databáze:	MEDLINE
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