Associations Between Prenatal Vitamin D and Placental Gene Expression.

Autor:	Parenti M; Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA, United States., Melough MM; Department of Health Behavior and Nutrition Sciences, University of Delaware, Newark, DE, United States., Lapehn S; Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA, United States., MacDonald J; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, United States., Bammler T; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, United States., Firsick EJ; Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA, United States., Choi HY; Department of Preventive Medicine, University of Tennessee Health Sciences Center, Memphis, TN, United States., Derefinko KJ; Department of Preventive Medicine, University of Tennessee Health Sciences Center, Memphis, TN, United States.; Department of Pharmacology, Addiction Science, and Toxicology, University of Tennessee Health Sciences Center, Memphis, TN, United States., Enquobahrie DA; Department of Epidemiology, University of Washington, Seattle, WA, United States., Carroll KN; Icahn School of Medicine at Mount Sinai, New York, NY, United States., LeWinn KZ; Department of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, CA, United States., Bush NR; Department of Psychiatry and Behavioral Sciences, University of California, San Francisco, San Francisco, CA, United States.; Department of Pediatrics, University of California, San Francisco, San Francisco, CA, United States., Zhao Q; Department of Preventive Medicine, University of Tennessee Health Sciences Center, Memphis, TN, United States., Sathyanarayana S; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, United States.; Department of Epidemiology, University of Washington, Seattle, WA, United States.; Center for Child Health, Behavior, and Development, Seattle Children's Research Institute, Seattle, WA, United States.; Department of Pediatrics, University of Washington, Seattle, WA, United States., Paquette AG; Center for Developmental Biology and Regenerative Medicine, Seattle Children's Research Institute, Seattle, WA, United States.; Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, WA, United States.; Department of Pediatrics, University of Washington, Seattle, WA, United States.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 May 12. Date of Electronic Publication: 2024 May 12.
DOI:	10.1101/2024.05.10.593571
Abstrakt:	Background: Vitamin D is a hormone regulating gene transcription. Prenatal vitamin D has been linked to immune and vascular function in the placenta, a key organ of pregnancy. To date, studies of vitamin D and placental gene expression have focused on a limited number of candidate genes. Transcriptome-wide RNA sequencing can provide a more complete representation of the placental effects of vitamin D. Objective: We investigated the association between prenatal vitamin D levels and placental gene expression in a large, prospective pregnancy cohort. Methods: Participants were recruited in Shelby County, Tennessee in the Conditions Affecting Neurocognitive Development and Learning in Early childhood (CANDLE) study. Vitamin D level (plasma total 25-hydroxyvitatmin D, [25(OH)D]) was measured at mid-pregnancy (16-28 weeks' gestation) and delivery. Placenta samples were collected at birth. RNA was isolated and sequenced. We identified differentially expressed genes (DEGs) using adjusted linear regression models. We also conducted weighted gene co-expression network analysis (WGCNA). Results: The median 25(OH)D of participants was 21.8 ng/mL at mid-pregnancy ( N =774, IQR: 15.4-26.5 ng/mL) and 23.6 ng/mL at delivery ( N =753, IQR: 16.8-29.1 ng/mL). Placental expression of 25 DEGs was associated with 25(OH)D at mid-pregnancy, but no DEG was associated with 25(OH)D at delivery. DEGs were related to energy metabolism, cytoskeletal function, and RNA transcription. Using WGCNA, we identified 2 gene modules whose expression was associated with 25(OH)D at mid-pregnancy and 1 module associated with 25(OH)D at delivery. These modules were enriched for genes related to mitochondrial and cytoskeletal function, and were regulated by transcription factors including ARNT2 , BHLHE40 , FOSL2 , JUND , and NFKB1 . Conclusions: Our results indicate that 25(OH)D during mid-pregnancy, but not at delivery, is associated with placental gene expression at birth. Future research is needed to investigate a potential role of vitamin D in programming placental mitochondrial metabolism, intracellular transport, and transcriptional regulation during pregnancy.
Databáze:	MEDLINE
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