Homeostatic T helper 17 cell responses triggered by complex microbiota are maintained in ex vivo intestinal tissue slices.

Autor: Beneke V; Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.; Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Research Network, Hannover, Germany.; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany., Grieger KM; Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.; Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Research Network, Hannover, Germany.; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany., Hartwig C; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Department of In-vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany., Müller J; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Department of In-vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany.; Center of Integrative Bioinformatics Vienna (CIBIV), Max Perutz Labs, University of Vienna and Medical University of Vienna, Vienna BioCenter, Vienna, Austria.; Member of the Vienna Biocenter PhD Program, University of Vienna and the Medical University of Vienna, Vienna, Austria., Sohn K; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Department of In-vitro Diagnostics, Fraunhofer Institute for Interfacial Engineering and Biotechnology, Stuttgart, Germany., Blaudszun AR; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Department of Cell and Gene Therapy Development, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany., Hilger N; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Department of Cell and Gene Therapy Development, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany., Schaudien D; Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany., Fricke S; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Department of Cell and Gene Therapy Development, Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany., Braun A; Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.; Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Research Network, Hannover, Germany.; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.; Institute for Immunology, Hannover Medical School, Hannover, Germany., Sewald K; Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.; Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Research Network, Hannover, Germany.; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany., Hesse C; Division of Preclinical Pharmacology and Toxicology, Fraunhofer Institute for Toxicology and Experimental Medicine, Hannover, Germany.; Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH) Research Network, Hannover, Germany.; Member of the Fraunhofer Excellence Cluster of Immune Mediated Diseases (CIMD), Germany.
Jazyk: angličtina
Zdroj: European journal of immunology [Eur J Immunol] 2024 Aug; Vol. 54 (8), pp. e2350946. Date of Electronic Publication: 2024 May 19.
DOI: 10.1002/eji.202350946
Abstrakt: Segmented filamentous bacteria (SFB) are members of the commensal intestinal microbiome. They are known to contribute to the postnatal maturation of the gut immune system, but also to augment inflammatory conditions in chronic diseases such as Crohn's disease. Living primary tissue slices are ultrathin multicellular sections of the intestine and provide a unique opportunity to analyze tissue-specific immune responses ex vivo. This study aimed to investigate whether supplementation of the gut flora with SFB promotes T helper 17 (Th17) cell responses in primary intestinal tissue slices ex vivo. Primary tissue slices were prepared from the small intestine of healthy Taconic mice with SFB-positive and SFB-negative microbiomes and stimulated with anti-CD3/CD28 or Concanavalin A. SFB-positive and -negative mice exhibited distinct microbiome compositions and Th17 cell frequencies in the intestine and complex microbiota including SFB induced up to 15-fold increase in Th17 cell-associated mediators, serum amyloid A (SAA), and immunoglobulin A (IgA) responses ex vivo. This phenotype could be transmitted by co-housing of mice. Our findings highlight that changes in the gut microbiome can be observed in primary intestinal tissue slices ex vivo. This makes the system very attractive for disease modeling and assessment of new therapies.
(© 2024 The Authors. European Journal of Immunology published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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