Virulence factors, antifungal susceptibility and molecular profile in Candida species isolated from the hands of health professionals before and after cleaning with 70% ethyl alcohol-based gel.

Autor: Alves PGV; Postgraduate Program in Health Sciences, Medicine, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil., Menezes RP; Technical School of Health, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil., Silva NBS; Postgraduate Program in Applied Immunology and Parasitology, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil., Faria GO; Biologist, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Bessa MAS; Faculty of Mathematics, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil., de Araújo LB; Clinical Hospital, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil., Aguiar PADF; Biologist, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil., Penatti MPA; Technical School of Health, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil., Pedroso RDS; Technical School of Health, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil. Electronic address: rpedroso@ufu.br., Röder DVDB; Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil.
Jazyk: angličtina
Zdroj: Journal de mycologie medicale [J Mycol Med] 2024 Jun; Vol. 34 (2), pp. 101482. Date of Electronic Publication: 2024 May 09.
DOI: 10.1016/j.mycmed.2024.101482
Abstrakt: Fungal infections in neonatal intensive care units (NICU) are mainly related to Candida species, with high mortality rates. They are predominantly of endogenous origin, however, cross-infection transmitted by healthcare professionals' hands has occurred. The aim of this study was to identify Candida species isolated from the hands of healthcare professionals in a NICU before and after hygiene with 70% ethanol-based gel and evaluate virulence factors DNase, phospholipase, proteinase, hemolysin, biofilm biomass production, and metabolic activity. In vitro antifungal susceptibility testing and similarity by random amplified polymorphic DNA (RAPD) were also performed. C. parapsilosis complex was the most frequent species (57.1%); all isolates presented at least one virulence factor; three isolates (Candida parapsilosis complex) were resistant to amphotericin B, two (Candida famata [currently Debaryomyces hansenii] and Candida guilliermondii [currently Meyerozyma guilliermondii]) was resistant to micafungin, and six (Candida parapsilosis complex, Candida guilliermondii [=Meyerozyma guilliermondii], Candida viswanathi, Candida catenulata [currently Diutina catenulata] and Candida lusitaniae [currently Clavispora lusitaniae]) were resistant to fluconazole. Molecular analysis by RAPD revealed two clusters of identical strains that were in the hands of distinct professionals. Candida spp. were isolated even after hygiene with 70% ethanol-based gel, highlighting the importance of stricter basic measures for hospital infection control to prevent nosocomial transmission.
Competing Interests: Declaration of competing interest none.
(Copyright © 2024. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
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