Protocol to culture enteric glial cells from the submucosal and myenteric plexi of neonatal and juvenile pig colons.
| Autor: | Caldwell ML; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA., Cook CA; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA., Mariant CL; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA., Touvron M; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA., Odle J; Department of Animal Sciences, College of Agricultural and Life Sciences, North Carolina State University, Raleigh, NC 27603, USA., Blikslager AT; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA., Ziegler AL; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA., Van Landeghem L; Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27603, USA. Electronic address: lcvanlan@ncsu.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2024 Jun 21; Vol. 5 (2), pp. 103057. Date of Electronic Publication: 2024 May 18. |
| DOI: | 10.1016/j.xpro.2024.103057 |
| Abstrakt: | Here, we present our protocol to culture enteric glial cells from the submucosal and myenteric plexus of neonatal and juvenile pig colons. We describe steps for colon isolation, microdissection, and enzymatic and mechanical dissociation. We include procedures for passaging and analyzing cell yield, freeze/thaw efficiency, and purity. This protocol allows for the generation of primary cultures of enteric glial cells from single-cell suspensions of microdissected layers of the colon wall and can be used to culture enteric glia from human colon specimens. For complete details on the use and execution of this protocol, please refer to Ziegler et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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