Applying a heat transfer mathematical model for the cryopreservation of rainbow trout (Oncorhynchus mykiss) sperm: How straw location over liquid nitrogen level affects freezing rate and fertilization yield.

Autor: Santos MV; Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales (IPATEC), Universidad Nacional Del Comahue (UNCO) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Quintral 1250, Bariloche, 8400, Río Negro, Argentina. Electronic address: mvsantos@comahue-conicet.gob.ar., Crichigno SA; Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales (IPATEC), Universidad Nacional Del Comahue (UNCO) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Quintral 1250, Bariloche, 8400, Río Negro, Argentina., Cussac VE; Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales (IPATEC), Universidad Nacional Del Comahue (UNCO) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Quintral 1250, Bariloche, 8400, Río Negro, Argentina., Zaritzky N; Centro de Investigación y Desarrollo de Criotecnología de Alimentos (CIDCA), CONICET - Departamento de Ing. Química, Facultad de Ingeniería, Universidad Nacional de La Plata, CIC-PBA, 115 y 47 La Plata, 1900, Buenos Aires, Argentina.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2024 Jun; Vol. 115, pp. 104908. Date of Electronic Publication: 2024 May 27.
DOI: 10.1016/j.cryobiol.2024.104908
Abstrakt: Cryopreservation of rainbow trout semen under field conditions was analyzed. Straw location over liquid nitrogen level is a crucial variable that affects freezing rate and fertilization yield due to changes in nitrogen vapor external temperature. The objectives were: to analyze cryopreservation protocols by experimentally measuring the cooling rates and fertilization yield of 0.5 ml plastic straws located in nitrogen vapor at different heights corresponding to different external temperatures; to numerically simulate the freezing process, by solving the heat transfer partial differential equations with the corresponding thermo-physical properties of the biological system and the plastic straw; to evaluate and analyze the surface heat transfer coefficient (h) during the freezing process of the straws; to introduce a new variable, the characteristic freezing time (tc), that enables comparison between protocols; this variable was defined as the elapsed period between the initial freezing temperature and a final reference temperature of -40 °C (temperature in which more than 80 % of the water is in a frozen state). The mathematical model predicted the temperature distribution inside the straw, showing a low effect of straw plastic materials (polyethylene-terephthalate glycol, polyvinyl-chloride, and polypropylene) on freezing rates. The average h value obtained from numerical simulations was 25.5 W/m 2 K, close to that obtained from the analytical Nusselt correlation for natural convection. An improvement on fertilization trials was observed when the average external nitrogen temperature was -129.6 °C (temperature range: -94 to -171 °C) with an average tc of 56.8 s (ranging between 47 and 72 s). These results corresponded to a height above the level of liquid nitrogen of 2 cm. Comparison with literature reported data showed satisfactory results. Applying mathematical models in the cryobiology field achieved results that are relevant for cryopreservation activities.
Competing Interests: Declaration of competing interest Authors declare no conflict of interest in the present study.
(Copyright © 2024 Society for Cryobiology. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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