Unraveling hallmark suitability for staging pre- and post-implantation stem cell models.

Autor:	Onfray C; Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France., Chevolleau S; Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France., Moinard E; Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France., Girard O; Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France., Mahadik K; Université Paris Cité, CNRS, Epigenetics and Cell Fate, 75013 Paris, France., Allsop R; KU Leuven - University of Leuven, Department of Development and Regeneration, Leuven Institute for Single Cell Omics and Leuven Stem Cell Institute, Herestraat 49, 3000 Leuven, Belgium., Georgolopoulos G; KU Leuven - University of Leuven, Department of Development and Regeneration, Leuven Institute for Single Cell Omics and Leuven Stem Cell Institute, Herestraat 49, 3000 Leuven, Belgium., Lavigne R; University Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail) - UMR_S 1085, 35000 Rennes, France; University Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, Protim Core Facility, 35000 Rennes, France., Renoult O; Nantes Université, CNRS, Inserm, CRCI2NA, 44000 Nantes, France., Aksoy I; University Lyon, Université Lyon 1, Inserm, Stem Cell and Brain Research Institute U1208, 69500 Bron, France., Lemaitre E; Nantes Université, CHU Nantes, Inserm, CNRS, BioCore, SFR Bonamy, 44000 Nantes, France., Hulin P; Nantes Université, CHU Nantes, Inserm, CNRS, BioCore, SFR Bonamy, 44000 Nantes, France., Ouimette JF; Université Paris Cité, CNRS, Epigenetics and Cell Fate, 75013 Paris, France., Fréour T; Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France; Department of Obstetrics, Gynecology and Reproductive Medicine, Dexeus University Hospital, 08028 Barcelona, Spain; CHU Nantes, Service de Biologie de la Reproduction, 44000 Nantes, France., Pecqueur C; Nantes Université, CNRS, Inserm, CRCI2NA, 44000 Nantes, France., Pineau C; University Rennes, Inserm, EHESP, Irset (Institut de Recherche en Santé, Environnement et Travail) - UMR_S 1085, 35000 Rennes, France; University Rennes, CNRS, Inserm, Biosit UAR 3480 US_S 018, Protim Core Facility, 35000 Rennes, France., Pasque V; KU Leuven - University of Leuven, Department of Development and Regeneration, Leuven Institute for Single Cell Omics and Leuven Stem Cell Institute, Herestraat 49, 3000 Leuven, Belgium., Rougeulle C; Université Paris Cité, CNRS, Epigenetics and Cell Fate, 75013 Paris, France., David L; Nantes Université, CHU Nantes, Inserm, CR2TI, 44000 Nantes, France; Nantes Université, CHU Nantes, Inserm, CNRS, BioCore, SFR Bonamy, 44000 Nantes, France. Electronic address: laurent.david@univ-nantes.fr.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2024 May 28; Vol. 43 (5), pp. 114232. Date of Electronic Publication: 2024 May 17.
DOI:	10.1016/j.celrep.2024.114232
Abstrakt:	The advent of novel 2D and 3D models for human development, including trophoblast stem cells and blastoids, has expanded opportunities for investigating early developmental events, gradually illuminating the enigmatic realm of human development. While these innovations have ushered in new prospects, it has become essential to establish well-defined benchmarks for the cell sources of these models. We aimed to propose a comprehensive characterization of pluripotent and trophoblastic stem cell models by employing a combination of transcriptomic, proteomic, epigenetic, and metabolic approaches. Our findings reveal that extended pluripotent stem cells share many characteristics with primed pluripotent stem cells, with the exception of metabolic activity. Furthermore, our research demonstrates that DNA hypomethylation and high metabolic activity define trophoblast stem cells. These results underscore the necessity of considering multiple hallmarks of pluripotency rather than relying on a single criterion. Multiplying hallmarks alleviate stage-matching bias. Competing Interests: Declaration of interests The authors declare no conflict of interest. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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