A Solanum lycopersicum polyamine oxidase contributes to the control of plant growth, xylem differentiation, and drought stress tolerance.

Autor: D'Incà R; Department of Science, University Roma Tre, 00146, Rome, Italy., Mattioli R; Department of Science, University Roma Tre, 00146, Rome, Italy., Tomasella M; Dipartimento di Scienze della Vita, Università di Trieste, Trieste, Italy., Tavazza R; Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), BIOAG-BIOTEC C.R. Casaccia, Rome, Italy., Macone A; Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Incocciati A; Department of Biochemical Sciences 'A. Rossi Fanelli', Sapienza University of Rome, Rome, Italy., Martignago D; Department of Biosciences, University of Milano, Milan, Italy., Polticelli F; Department of Science, University Roma Tre, 00146, Rome, Italy.; National Institute of Nuclear Physics, Roma Tre Section, 00146, Rome, Italy., Fraudentali I; Department of Science, University Roma Tre, 00146, Rome, Italy., Cona A; Department of Science, University Roma Tre, 00146, Rome, Italy.; Istituto Nazionale Biostrutture e Biosistemi (INBB), Rome, Italy., Angelini R; Department of Science, University Roma Tre, 00146, Rome, Italy.; Istituto Nazionale Biostrutture e Biosistemi (INBB), Rome, Italy.; NBFC, National Biodiversity Future Center, Palermo, Italy., Tavazza M; Italian National Agency for New Technologies, Energy and Sustainable Economic Development (ENEA), BIOAG-BIOTEC C.R. Casaccia, Rome, Italy., Nardini A; Dipartimento di Scienze della Vita, Università di Trieste, Trieste, Italy., Tavladoraki P; Department of Science, University Roma Tre, 00146, Rome, Italy.; Istituto Nazionale Biostrutture e Biosistemi (INBB), Rome, Italy.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Jul; Vol. 119 (2), pp. 960-981. Date of Electronic Publication: 2024 May 18.
DOI: 10.1111/tpj.16809
Abstrakt: Polyamines are involved in several plant physiological processes. In Arabidopsis thaliana, five FAD-dependent polyamine oxidases (AtPAO1 to AtPAO5) contribute to polyamine homeostasis. AtPAO5 catalyzes the back-conversion of thermospermine (T-Spm) to spermidine and plays a role in plant development, xylem differentiation, and abiotic stress tolerance. In the present study, to verify whether T-Spm metabolism can be exploited as a new route to improve stress tolerance in crops and to investigate the underlying mechanisms, tomato (Solanum lycopersicum) AtPAO5 homologs were identified (SlPAO2, SlPAO3, and SlPAO4) and CRISPR/Cas9-mediated loss-of-function slpao3 mutants were obtained. Morphological, molecular, and physiological analyses showed that slpao3 mutants display increased T-Spm levels and exhibit changes in growth parameters, number and size of xylem elements, and expression levels of auxin- and gibberellin-related genes compared to wild-type plants. The slpao3 mutants are also characterized by improved tolerance to drought stress, which can be attributed to a diminished xylem hydraulic conductivity that limits water loss, as well as to a reduced vulnerability to embolism. Altogether, this study evidences conservation, though with some significant variations, of the T-Spm-mediated regulatory mechanisms controlling plant growth and differentiation across different plant species and highlights the T-Spm role in improving stress tolerance while not constraining growth.
(© 2024 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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